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Mutual interaction between presynaptic α2-adrenoceptors and 5-HT1B receptors on the sympathetic nerve terminals of the rat inferior vena cava

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Summary

Segments of the rat vena cava preincubated with [3H]noradrenaline and superfused with [3H]noradrenaline-free solution containing desipramine and corticosterone were stimulated electrically (standard parameters: 150 mA, 0.3 ms, 0.66 Hz; duration 6 min). In some experiments the stimulation parameters were modified in order to obtain similar absolute release values despite the presence of an α-adrenoceptor agonist or antagonist or of 5-hydroxytryptamine (5-HT).

In a first set of experiments, the vascular segments were first exposed to an α-adrenoceptor ligand, which was kept present throughout the remainder of superfusion, and then to 5-HT. The release-inhibiting effect of 5-HT was attenuated by the α2-adrenoceptor agonists clonidine and B-HT 920 whereas it was enhanced by the α-adrenoceptor antagonists phentolamine and idazoxan. The α1-adrenoceptor antagonist prazosin did not change the 5-HT-induced inhibition of noradrenaline (NA) release. In a second set of experiments, 5-HT was administered first and kept present in the superfusion fluid for the remainder of the experiment. In the presence of 5-HT, the overflow-inhibiting effects of B-HT 920 and clonidine and the overflow-enhancing effect of idazoxan were reduced.

The results demonstrate that activation of one kind of receptor decreased the inhibition of noradrenaline release produced by activation of the other. These effects were not the consequence of the change of release per se induced by the interacting drugs, since they also occurred when the release was adjusted to similar levels by modification of the stimulation parameters. Therefore, the effects are probably due to mutual interaction between presynaptic α2-adrenoceptors and 5-HT1B receptors. The level (receptors themselves, G proteins and/or ion channels) at which this interaction occurs is unknown.

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  1. Institut für Pharmakologie und Toxikologie der Universität Bonn, Reuterstrasse 2b, D-5300, Bonn 1, Federal Republic of Germany

    G. J. Molderings & M. Göthert

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  1. G. J. Molderings
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  2. M. Göthert
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Molderings, G.J., Göthert, M. Mutual interaction between presynaptic α2-adrenoceptors and 5-HT1B receptors on the sympathetic nerve terminals of the rat inferior vena cava. Naunyn-Schmiedeberg's Arch Pharmacol 341, 391–397 (1990). https://doi.org/10.1007/BF00176329

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  • DOI: https://doi.org/10.1007/BF00176329

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