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Comparison of carbon metabolism between chloramphenicol-treated and untreated cultures of Clostridium acetobutylicum

  • Applied Microbial and Cell Physiology
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Summary

Carbon distribution from substrates to products in Clostridium acetobutylicum ATCC 824 was investigated by adding 14C-labeled substrates as tracers. Comparison of carbon conversion between chloramphenicol (CAP)-treated and untreated cultures was also studied. The percentage of 14C recovery in butanol, acetone and ethanol from uniformly labeled [14C]glucose was increased by 17, 25 and 30%, respectively, after CAP addition. The incorporation of 14C in solvents from 14C-labeled acetate and butyrate was also increased by the antibiotic treatment. A total 14C recovery of 12% in all the products from added [14C]Na2CO3 indicates significant heterotrophic CO2 fixation in this microorganism. The ratio of carbon in butanol derived from glucose, acetate and butyrate was about 71:6:18, and this ratio was unchanged by CAP treatment.

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Author notes

  1. Xiangdong Zhou

    Present address: Buckman Laboratories International, Inc., 1245 N. McLean Boulevard, 38108, Memphis, TN, USA

Authors and Affiliations

  1. Department of Food Science and Nutrition, University of Rhode Island, 02881, Kingston, RI, USA

    Xiangdong Zhou & Richard W. Traxler

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  1. Xiangdong Zhou
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  2. Richard W. Traxler
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Additional information

This paper represents contribution No. 2685 of the Rhode Island Agricultural Experimental Station

Correspondence to: R. W. Traxler

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Zhou, X., Traxler, R.W. Comparison of carbon metabolism between chloramphenicol-treated and untreated cultures of Clostridium acetobutylicum . Appl Microbiol Biotechnol 38, 254–258 (1992). https://doi.org/10.1007/BF00174478

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  • DOI: https://doi.org/10.1007/BF00174478

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