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Expression and organization of muscle specific proteins during the early developmental stages of the rabbit heart

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Summary

The expression and intracellular distribution patterns of muscle-specific proteins were studied during rabbit embryo development (7–13 dpc) using monoclonal antibodies against titin, myosin, tropomyosin and actin, as well as the intermediate filament proteins desmin, keratin and vimentin. From our panel, titin appeared to be the first muscle-specific protein to be exclusively expressed in the embryonic rabbit heart. Upon differentiation (myocyte and myotube formation), titin reorganizes from dot-like aggregates into a cross-striated pattern (in 9- to 30-somite embryos) via a transiently filamentous distribution. When the expression and organization of the other muscle proteins was studied in relation to titin, it became apparent that tropomyosin followed upon titin with respect to its exclusive expression in the heart anlagen and its organization into a striated pattern. Myosin and desmin were organized into cross-striated patterns after titin and tropomyosin, but this arrangement had not reached its final form in 13-dpc embryos. Actin, keratin and vimentin were distributed in cytoplasmic filaments in the embryonic stages we investigated. Since the first pulsations are already detected in 3-somite embryos, we conclude that the organization of titin, tropomyosin, myosin and desmin into a striated pattern does not seem to be essential for the initiation of muscle cell contraction in the heart anlagen. Furthermore, this study shows that, in comparison with studies on mouse, chick and rat, the sequence of expression of muscle-specific and intermediate filament proteins during cardiomyogenesis is species-dependent, and that their expression and organization varies in time in different regions of the developing heart.

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Abbreviations

IFP :

intermediate filament proteins

PBS :

phosphate-buffered saline

FITC :

fluorescein isothiocyanate

TRITC :

tetramethylrhodamine isothiocyanate

TxRd :

texas red

dpc :

days post conception

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Authors and Affiliations

  1. Department of Molecular Cell Biology & Genetics, Cardiovascular Research Institute, University of Limburg, P.O. Box 616, NL-6200, MD Maastricht, The Netherlands

    F. T. L. van der Loop, G. Schaart & F. C. S. Ramaekers

  2. Institute of Anatomy, University of Bonn, W-5300, Bonn, Federal Republic of Germany

    W. Langmann & Ch. Viebahn

Authors
  1. F. T. L. van der Loop
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  2. G. Schaart
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  3. W. Langmann
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  4. F. C. S. Ramaekers
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  5. Ch. Viebahn
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van der Loop, F.T.L., Schaart, G., Langmann, W. et al. Expression and organization of muscle specific proteins during the early developmental stages of the rabbit heart. Anat Embryol 185, 439–450 (1992). https://doi.org/10.1007/BF00174082

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