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Distribution patterns of dendrites in motor neuron pools of lumbosacral spinal cord of the chicken

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Summary

The morphology of dendritic trees (dendroarchitecture) of motor neurons innervating specific hindlimb muscles (motoneuron pools, MNP) was studied in the chick spinal cord. Motoneurons were labelled by intramuscular injections of horseradish peroxidase conjugated with cholera toxin subunit B. MNPs of posterior iliotibial and femorotibial muscles were located at the dorsolateral part of lateral motor column of lumbosacral segments (LS) 1–4 and 1–3, respectively. Although the dendritic profiles of femorotibialis motoneurons were fewer than those of posterior iliotibialis, these two MNPs had a similar distribution pattern of dendrites. Dendritic profiles were about equally distributed in the gray and white matter. Dendrites from the MNP of posterior iliotibialis radiated in all directions. A large number of dendrites penetrated into the white matter, and some even reached to the subpial regions of the lateral funiculus. One array of dendrites that projected dorsomedialwards extended to the base of the posterior horn. MNPs of both the iliofibularis (LS 4–7) and caudilioflexorius (LS 6–8) had dendritic trees with similar distribution patterns. There were two main arrays of dendritic extensions; one along the dorsal, and another along the ventral border of the lateral motor column. Dendrites from the iliofibularis and caudilioflexorius motoneurons were located more frequently in the white matter than in the gray matter. A large number of dendrites extended in all directions from the MNP of the adductor muscle, which was located in the medial region of lateral motor column of LS 1–2. The distribution of dendrites from a few other MNPs was also examined. From these observations, we conclude that there are major differences in the distribution of dendrites of MNPs innervating different chick hind limb muscles. We discuss the possibility that these differences may be associated with differences in the quantity or quality of afferent inputs received by motoneurons in the various MNPs.

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References

  • Aitken JT. Bridger JE (1961) Neuron size and neuron population density in the lumbosacral region of the cat's spind cord. J Anat 95:38–53

    Google Scholar 

  • Abdel-Maguid TE, Bowsher D (1979) Alpha- and gamma-motoneurons in the adult human spinal cord and somatic cranial nerve nuclei: The significance of dendroarchitectonics studied by the Golgi method. J Comp Neurol 186:259–270

    Google Scholar 

  • Barrett JN, Crill WE (1971) Specific membrane resistivity of dye-injected motoneurons. Brain Res 28:556–561

    Google Scholar 

  • Barrett JN, Crill WE (1974) Specific membrane properties of cat motoneurons. J Physiol 239:301–324

    Google Scholar 

  • Bellinger DL, Anderson WJ (1987) Postnatal development of cell columns and their associated dendritic bundles in the lumbosacral spinal cord of the rat. II. The ventrolateral cell column. Dev Brain Res 35:69–82

    Google Scholar 

  • Berger AJ, Cameron WE, Averill DB, Kramis RC, Binder MD (1984) Spacial distribution of phrenic and medial gastrocnemius motoneurons in the cat spinal cord. Exp Neurol 86:559–575

    Google Scholar 

  • Bernstein JJ, Standler N (1983) Dendritic alternation of rat spinal motoneurons after dorsal horn mince: computer reconstruction of dendritic fields. Exp Neurol 82:532–540

    Google Scholar 

  • Bregman B, Cruce WLR (1980) Normal dendritic morphology of frog spinal motoneurons: A Golgi study. J Comp Neurol 193:1035–1045

    Google Scholar 

  • Cabot JB, Reiner A, Bogan N (1982) Avian bulbospinal pathways: Anterograde studies of cells of origin, funicular trajectories and laminar terminations. In: Kuypers HGJM, Martin GF (eds) Progress in Brain Research, vol 57. Descending pathways to the spinal cord, Elsevier

  • Crone C, Hultborn H, Kiehn O, Mazieres L, Wigstrom H (1988) Maintained changes in motoneuronal excitability by short-lasting synaptic inputs in the decerebrate cat. J Physiol 405:321–343

    Google Scholar 

  • Cruce WLR (1974) The anatomical organization of hindlimb motoneurons in the lumbar spinal cord of the frog, Rana catesbiana. J Comp Neurol 153:59–76

    Google Scholar 

  • Cullheim S, Fleshman JW, Glenn LL, Burke RE (1987a) Membrane area and dendritic structure in type-identified triceps surae alpha motoneurons. J Comp Neurol 255:68–81

    Google Scholar 

  • Cullheim S, Fleshman JW, Glenn LL, Burke RE (1987b) Threedimensional architecture of trees in type-identified-motoneurons. J Comp Neurol 255:82–96

    Google Scholar 

  • Das GD (1975) Differentiation of dendrites in transplanted neuroblast in the mammalian brain. Adv Neurol 12:181–199

    Google Scholar 

  • Deitch JS, Rubel EW (1984) Afferent influences on brain stem auditory nuclei of the chicken: Time course and specificity of dendritic atrophy following deafferentation. J Comp Neurol 229:66–79

    Google Scholar 

  • Egger MD, Freeman NCG, Proshansky E (1980) Morphology of spinal motoneurones mediating a cutaneous spinal reflex in the cat. J Physiol 306:349–367

    Google Scholar 

  • English AW (1984) An electromyographic analysis of compartments in cat lateral gastrocnemius muscle during unrestrained locomotion. J Neurophysiol 52:114–125

    Google Scholar 

  • Fetcho JR (1986) The organization of the motoneurons innervating the axial musculature of vertebrates. 1. Goldfish (Carassius aur-atus) and mudpuppies (Necturus maculosus). J Comp Neurol 249:521–550

    Google Scholar 

  • Furicchia JV, Goshgarian HG (1987) Dendritic organization of phrenic motoneurons in the adult rat. Exp Neurol 96:621–634

    Google Scholar 

  • Haase P, Hrycyshyn AW (1986) On the diffusion of horseradish peroxidase into muscles and the “spurious” labelling of motoneurons. Exp Neurol 91:399–403

    Google Scholar 

  • Hardman VJ, Brown MC (1985) Spacial organization within rat motoneuron pools. Neurosci Lett 60:325–329

    Google Scholar 

  • Hollyday M (1980) Organization of motor pools in the chick lumbar lateral motor column. J Comp Neurol 194:143–170

    Google Scholar 

  • Holstege B, Blok BF, Ralston DD (1988) Anatomical evidence for red nucleus projections to motoneuronal cell groups in the spinal cord of the monkey. Neurosci Lett 95:97–101

    Google Scholar 

  • Homma S, Sako H, Kohno K, Okado N (1988) The pattern of distribution of serotoninergic fibers in the anterior horn of the chick spinal cord. Anat Embryol 179:25–31

    Google Scholar 

  • Hongo T, Kudo N, Sasaki N, Yamashita M, Yoshida K, Ishizuka N, Mannen H (1987) Trajectory of group Ia and Ib fibers from the hind-limb muscles at the L3 and L4 segments of the spinal cord of the cat. J Comp Neurol 262:159–195

    Google Scholar 

  • Hounsgaard J, Hultborn H, Jespersen B, Kiehn O (1988) Bistability of α-motoneurones in the decerebrate cat and in the acute spinal cat after intravanous 5-hydroxytryptophan. J Physiol 405:345–367

    Google Scholar 

  • Ishizuka N, Mannen H, Hongo T, Sasaki N (1979) Trajectory of group Ia afferent fibers stained with horseradish peroxidase in the lumbosacral spinal cord of the cat: Three dimensional reconstructions from serial sections. J Comp Neurol 186:189–212

    Google Scholar 

  • Jacobson RD, Hollyday M (1982a) A behavioral and electromyographic study of walking in the chick. J Neurophysiol 48:238–256

    Google Scholar 

  • Jacobson RD, Hollyday M (1982b) Electrically evoked walking and fictive locomotion in the chick. J Neurophysiol 48:257–270

    Google Scholar 

  • Jankowska E, Rastad J, Westman J (1976) Intracellular application of horseradish peroxidase and its light and electron microscopical appearance in spinocervical tract cells. Brain Res 105:557–562

    Google Scholar 

  • Kojima T, Homma S, Sako H, Shimizu I, Okada A, Okado N (1988) Developmental changes in density and distribution of serotoninergic fibers in the chick spinal cord. J Comp Neurol 267:580–589

    Google Scholar 

  • Kudo N, Yamada T (1987) Morphological and physiological studies of development of the monosynaptic reflex pathway in the rat lumbar spinal cord. J Physiol 389:441–459

    Google Scholar 

  • Kuypers HGJM (1982) A new look at the organization of the motor system. In: Kuypers HGJM, Martin GF (eds) Progress in Brain Research, vol 57. Descending pathways to the spinal cord. Elsevier, Amsterdam, pp 381–403

    Google Scholar 

  • Landmesser L (1978) The distribution of motoneurones supplying chick hind limb muscles. J Physiol 284:371–389

    Google Scholar 

  • Lichtman JF, Jhaveri S, Frank E (1984) Anatomical basis of specific connections between sensory axons and motor neurons in the brachial spinal cord of the bullfrog. J Neurosci 4:1754–1763

    Google Scholar 

  • Liddell EGT, Sherrington CS (1924) Reflexes in response to stretch (myotatic reflexes). Proc R Soc Lond [Biol] 96:212–242

    Google Scholar 

  • Liddell EGT, Sherrington CS (1925) Further observations on myotatic reflexes. Proc R Soc Lond [Biol] 97:276–283

    Google Scholar 

  • Lloyd DPC (1943) Conduction and synaptic transmission of reflex response to stretch in spinal cats. J Neurophysiol 6:317–326

    Google Scholar 

  • Lux HD, Schubert P (1975) Some aspects of the electroanatomy of dendrites. In: Kreutzberg GW (ed) Advances in Neurology, vol 12. Raven Press, New York, pp 29–44

    Google Scholar 

  • Martin AH (1979) A cytoarchitectonic scheme for the spinal cord of the domestic fowl, Gallus gallus domesticus: Lumbar region. Acta Morphol Neerl Scand 17:105–117

    Google Scholar 

  • Martin AH, Hrycyshyn AW (1981) Neurons for flight, a horseradish peroxidase study. Exp Neurol 72:252–256

    Google Scholar 

  • Mesulam M-M, Hegarty E, Barbas H, Carson KA, Grower EC, Knapp AG, Moss MB, Mufson EJ (1980) Additional factors influencing sensitivity in the tetramethyl benzidine method for horseradish peroxidase neurohistochemistry. J Histochem Cytochem 28:1255–1259

    Google Scholar 

  • Motorina MV (1974) Distribution and types of rubro-motoneuronal synapses in the lumbar segments of the cat. J Evol Biochem Physiol USSR 10:88–95

    Google Scholar 

  • Nicolopoulos-Stournaras S, Iles JF (1983) Motor neuron columns in the lumbar spinal cord of the rat. J Comp Neurol 217:75–85

    Google Scholar 

  • Ohmori Y, Watanabe T, Fujioka T (1984) Localizations of motoneurons innervating the hindlimb muscles in the spinal cord of the domestic fowl. Zentralbl Veterinarmed [C] 13:141–155

    Google Scholar 

  • Oka Y, Takeuchi H, Satou M, Ueda K (1987) Cobalt lysine study of the morphology and distribution of the cranial nerve efferent neurons (motoneurons and preganglionic parasympathetic neurons) and rostral spinal motoneurons in the Japanese toad. J Comp Neurol 259:400–423

    Google Scholar 

  • Okado N, Homma S, Ishihara R, Sako H, Kohno K (1988) Differential innervation of specific motor neuron pools by serotoninergic fibers in the chick spinal cord. Neurosci Lett 94:29–32

    Google Scholar 

  • Okado N, Ishihara R, Homma S, Ito R, Kohno K (1989) Immunohistochemical study on tyrosine hydroxylase positive cells and fibers in the chick spinal cord. Anat Embryol (submitted)

  • Oppenheim RW, Chu-Wang IW, Foelix RF (1975) Some aspects of synaptogenesis in the spinal cord of the chick embryo: A quantitative electron microscopic study. J Comp Neurol 161:383–418

    Google Scholar 

  • Ralston DD, Milroy AM, Holstege G (1988) Ultrastructural evidence for direct monosynaptic rubrospinal connections to motoneurons in Macaca mulatta. Neurosci Lett 95:102–106

    Google Scholar 

  • Robert LB, Cohen DH (1975) Spinal terminal fields of dorsal root fibers in the Pigeon (Columba livia). J Comp Neurol 163:181–192

    Google Scholar 

  • Romanes GJ (1964) The motor pools of the spinal cord. In: Eccles JC, Schadé JP (eds) Organization of the Spinal Cord, vol 11. Progress in Brain Research, pp 93–119

  • Rose PK (1981) Distribution of dendrites from biventer cervicis and complexus motoneurons stained intracellularly with horseradish peroxidase in the adult cat. J Comp Neurol 197:395–409

    Google Scholar 

  • Rose PK, Richmond FJR (1981) White-matter dendrites in the upper cervical spinal cord of the adult cat: A light and electron microscopic study. J Comp Neurol 199:191–203

    CAS  PubMed  Google Scholar 

  • Ruigrock TJH, Crowe A (1984) The organization of motoneurons in the turtle lumbar spinal cord. J Comp Neurol 228:24–37

    Google Scholar 

  • Ruigrock TJH, Crowe A, Ten Donkellaar HJ (1984) Morphology of lumbar motoneurons innervating hindlimb muscles in the turtle Pseudemys scripta elegans: An intracellular horseradish peroxidase study. J Comp Neurol 230:413–425

    Google Scholar 

  • Ruigrock TJH, Crowe A, Ten Donkellaar HJ (1985) Dendritic distribution of identified motoneurons in the lumbar spinal cord of the turtle Pseudemys scripta elegans. J Comp Neurol 238:275–285

    Google Scholar 

  • Sako H, Kojima T, Okado N (1986) Immunohistochemical study on the development of serotoninergic neurons in the chick: II. Distribution of cell bodies and fibers in the spinal cord. J Comp Neurol 253:79–91

    Google Scholar 

  • Schoenen J (1982) Dendritic organization of the human spinal cord: The motoneurons. J Comp Neurol 211:226–247

    Google Scholar 

  • Sharrard WJW (1955) The distribution of permanent paralysis in the lower limb in poliomyelitis. Br J Bone Joint Surg 37B:540–558

    Google Scholar 

  • Scheibel ME, Scheibel AB (1970) Developmental relationship between spinal motoneuron dendrite bundles and patterned activity in the hind limb of cats. Exp Neurol 29:328–335

    Google Scholar 

  • Shapovalov AI (1972) Extrapyramidal monosynaptic and disynaptic control of mammalian alpha-motoneurons. Brain Res 40:105–115

    Google Scholar 

  • Shapovalov AI (1975) Neural organization and synaptic mechanisms of supraspinal motor control in vertebrates. Rev Physiol Biochem Pharmacol 72:1–54

    Google Scholar 

  • Smith DE (1974) The effect of deafferentation on the postnatal development of Clarke's nucleus in the kitten — a Golgi study. Brain Res 74:119–130

    Google Scholar 

  • Smith CL (1983) The development and postnatal organization of primary afferent projections to the rat thoracic spinal cord. J Comp Neurol 220:29–43

    Google Scholar 

  • Snow PJ, Rose PK, Brown AG (1976) Tracing axons and axon collaterals of spinal neurons using intracellular injection of horseradish peroxidase. Science 191:312–313

    Google Scholar 

  • Standler NA, Bernstein JJ (1984) Dendritic alteration of spinal motoneurons after ablation of somatomotor cortex. Exp Neurol 83:264–273

    Google Scholar 

  • Steffen H, Van der Loos H (1980) Early lesions of mouse vibrissal follicles: their influence on dendritic orientation in the cortical barrel field. Exp Brain Res 40:419–431

    Google Scholar 

  • Straznickey C, Tay D (1983) The localization of motoneuron pools innervating wing muscles in the chick. Anat Embryol 166:209–218

    Google Scholar 

  • Szekely G (1976) The morphology of motoneurons and dorsal root fibers in the frog's spinal cord. Brain Res 103:275–290

    Google Scholar 

  • Trojanowski JQ, Gonatas JO, Gonatas NK (1982) Horseradish peroxidase (HRP) conjugates of cholera toxin and lectins are more sensitive retrogradely transported markers than free HRP. Brain Res 231:33–50

    Google Scholar 

  • Ulfhake B, Kellerth J-O (1981a) A quantitative light microscopic study of the dendrites of cat spinal α-motoneurons after intracellular staining with horseradish peroxidase. J Comp Neurol 202:571–583

    Google Scholar 

  • Ulfhake B, Kellerth J-O (1981b) A quantitative light microscopic study of the dendrites of cat spinal α-motoneurons after intracellular staining with horseradish peroxidase. J Comp Neurol 202:585–596

    Google Scholar 

  • Ulfhake B, Kellerth J-O (1983) A quantitative morphological study of HRP-labelled cat α-motoneurones supplying different hindlimb muscles. Brain Res 264:1–19

    Google Scholar 

  • Wan XST, Trojanowski JQ, Gonatas JO, Liu CN (1982) Cytoarchitecture of the extranuclear and commissural dendrites of hypoglossal nucleus neurons as revealed by conjugates of horse-radish peroxidase with cholera toxin. Exp Neurol 78:167–175

    Google Scholar 

  • Wild JM, Cabot JB, Cohen DH, Karten HJ (1979) Origin, course and terminations of the rubrospinal tract in the pigeon (Columba liva). J Comp Neurol 187:639–654

    Google Scholar 

  • Wold JE (1978) The vestibular nuclei in the domestic hen (Gallus domesticus). IV. The projection of the spinal cord. Brain Behav Evol 15:41–62

    Google Scholar 

  • Wolters JG, ten Donkelaar HJ, Verhofstad AAJ (1984) Distribution of catecholamines in the brain stem and spinal cord of the Lizard Veranus exanthematicus: An immunohistochemical study based on the use of antibodies to tyrosine hydroxylase. Neuroscience 13:469–497

    Google Scholar 

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  1. Shunsaku Homma

    Present address: Department of Neurobiology and Anatomy, Bowman Gray School of Medicine, 27103, Winston-Salem, NC, USA

Authors and Affiliations

  1. Department of Anatomy, University of Tsukuba, Institute of Basic Medical Sciences, Tsukuba, 305, Ibaraki, Japan

    Nobuo Okado, Shunsaku Homma, Rieko Ishihara & Kunio Kohno

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  1. Nobuo Okado
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  2. Shunsaku Homma
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Okado, N., Homma, S., Ishihara, R. et al. Distribution patterns of dendrites in motor neuron pools of lumbosacral spinal cord of the chicken. Anat Embryol 182, 113–121 (1990). https://doi.org/10.1007/BF00174012

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