Abstract
The suspension culture of high anthocyanin-producing Aralia cordata cell lines, which grow and produce anthocyanin without light irradation, was scaled up from flasks to a 10-1 glass jar fermentor, a 95-1 stainless steel jar fermentor, and finally a 500-l pilot-scale jar fermentor. By the administration of CO2, cell damage was completely prevented and the anthocyanin content was kept as high as 7.0–17.2% (w/w) of the dried cells. In one of the operations of the 500-l jar fermentor, cells were cultivated for 16 days. During this operation, cell mass was increased by more than 26 times (cell yield: 69.2 kg fresh wt.) and the amount of anthocyanin increased by more than 55 times (anthocyanin yield: 545 g, anthocyanin content: 17.2% of the dried cells).
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References
Callebaut A, Hendrickx G, Voets AM, Motte JC (1990) Anthocyanins in cell cultures of Ajuga reptans. Phytochemistry 29:2153–2158
Dougall DK, Johnson JM, Whitten GH (1980) A clonal analysis of anthocyanin-accumulation by cell cultures of wild carrot. Planta 149:292–297
Hara M, Kobayashi Y, Fukui H, Tabata M (1991) Enhancement of berberine production by spermidine in Thalictrum minus cell suspension cultures. Plant Cell Rep 10:494–497
Hiraoka N, Kodama T, Tomita Y (1986) Selection of Bupleurum falcatum callus line producing anthocyanins in darkness. J Nat Prod 49:470–474
Isenberg FMR (1979) Controlled atmosphere storage of vegetables. Hort Rev 1:337–394
Kinnersley AM, Dougall DK (1980) Increase in anthocyanin yield from wild-carrot cell cultures. Planta 149:200–204
Kobayashi Y, Fukui H, Tabata M (1991a) Effect of carbon dioxide and ethylene on berberine production and cell browning in Thalictrum minus cell cultures. Plant Cell Rep 9:496–499
Kobayashi Y, Hara M, Fukui H, Tabata M (1991b) The role of berberine production by spermidine in Thalictrum minus cell suspension cultures. Phytochemistry 30:3605–3609
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays. Physiol Plant 15:473–497
Sakamoto K, Iida K, Sawamura K, Hajiro K, Yoshikawa T, Furuya T (1993) Effects of nutrients on anthocyanin production in cultured cells of Aralia cordata. Phytochemistry 33:357–360
Smock RM (1979) Controlled atmosphere storage of fruits. Hort Rev 1:301–336
Street HE (1977) Cell (suspension) cultures-techniques. In: Street HE (ed) Plant tissue and cell culture. Blackwell, Oxford, pp 61–102
Yamakawa T, Ishida K, Kato S, Kodama T, Minoda Y (1983) Formation and identification of anthocyanins in cultured cells of Vitis sp. Agric Biol Chem 47:997–1001
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Correspondence to: Y. Kobayashi
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Kobayashi, Y., Akita, M., Sakamoto, K. et al. Large-scale production of anthocyanin by Aralia cordata cell suspension cultures. Appl Microbiol Biotechnol 40, 215–218 (1993). https://doi.org/10.1007/BF00170369
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DOI: https://doi.org/10.1007/BF00170369