Skip to main content

Characterization of a pyridine-degrading branched Gram-positive bacterium isolated from the anoxic zone of an oil shale column

Summary

From the anoxic zone of an oil shale leachate column three pyridine-degrading bacterial strains were isolated. Two strains were Gram-negative facultative anaerobic rods and one strain was a branched Gram-positive bacterium. The branched Gram-positive strain had the best pyridine-degrading ability. This organism was aerobic, non-motile, catalase positive, oxidase negative, and had no flagellum. The G+C content of the DNA was 66.5 mol%. The major menaquinone was MK-8(H2). The main cellular fatty acids were saturated and monounsaturated straight chains. This organism contained mycolic acid, meso-diaminopimelic acid, arabinogalactan and glycolyl residues in the cell wall. Due to morphological, physiological and chemotaxonomic characteristics this strain was placed in the genus Rhodococcus. The optimum culture conditions were as follows: temperature 32° C, pH 8.0 and 0.1% v/v of pyridine as sole carbon, energy and nitrogen source. Utilization of pyridine by a batch fermentor culture of Rhodococcus sp. was characterized by a specific growth rate of 0.13 h−1, growth yield of 0.61 mg cell·mg pyridine−1 and a doubling time of 5.3 h−1.

This is a preview of subscription content, access via your institution.

References

  • Becker B, Lechevalier MP, Gordon RE, Lechevalier HA (1964) Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Appl Microbiol 12:421–423

    Google Scholar 

  • Hugh R, Leifson E (1953) The aromatic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66:24–26

    Google Scholar 

  • Jori A, Calamari D, Cattabeni E, Domenico AD, Galli CL, Galli E, Silano V (1983) Ecotoxicological profile of pyridine. Ecotoxicol Environ Safety 7:251–275

    Google Scholar 

  • Kroppenstedt RM (1985) Fatty acid and menaquinone analysis of actinomycetes and related organism. Soc Appl Bacteriol Tech Ser 20:173–199

    Google Scholar 

  • Leenheer JA, Stuber HA (1981) Migration through soil of organic solutes in an oil-shale process water. Environ Sci Technol 15:1467–1475

    Google Scholar 

  • Mannikin DE, Hutchinson IG, Caldicott AB, Goodfellow M (1980) Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233

    Google Scholar 

  • Shukla OP, Kaul SM (1974) A constitutive pyridine degrading system in Corynebacterium sp. Indian J Biochem Biophysics 11:201–207

    Google Scholar 

  • Shukla OP, Kaul SM (1975) Succinate semialdehyde, an intermediate in the degradation of pyridine by Brevibacterium sp. Indian J Biochem Biophysics 12:326–330

    Google Scholar 

  • Sims GK, Sommers LE, Konopka A (1986) Degradation of pyridine by Micrococcus leteus isolated from soil. Appl Environ Microbiol 51:963–968

    Google Scholar 

  • Skerman VBD (1967) A guide to the identification of the genera of bacteria, 2nd edn. Williams and Wilkins, Baltimore

    Google Scholar 

  • Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231

    Google Scholar 

  • Stuermer DH, Ng DJ, Morris CJ (1982) Organic contaminants in groundwater near an underground coal gasification site in northeastern Wyoming. Environ Sci Technol 16:582–587

    Google Scholar 

  • Suzuki KI, Komagata K (1983) Taxonomic significance of cellular fatty acid composition in some coryneform bacteria. Int J Syst Bacteriol 33:188–200

    Google Scholar 

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  • Uchida K, Aida K (1979) Taxonomic significance of cell-wall acyl type in Corynebacterium-Mycobacterium-Nocarcia group by a glycolate test. J Gen Appl Microbiol 25:169–183

    Google Scholar 

  • Watson GK, Cain RB (1975) Microbial metabolism of the pyridine ring. Biochem J 146:157–172

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Offprint requests to: S.-T. Lee

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lee, ST., Lee, SB. & Park, YH. Characterization of a pyridine-degrading branched Gram-positive bacterium isolated from the anoxic zone of an oil shale column. Appl Microbiol Biotechnol 35, 824–829 (1991). https://doi.org/10.1007/BF00169903

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00169903

Keywords

  • Specific Growth Rate
  • Rhodococcus
  • Cellular Fatty Acid
  • Menaquinone
  • Mycolic Acid