Summary
The production of α-amylase by Bacillus licheniformis M27 in submerged fermentation was completely inhibited due to catabolic repression in medium containing 1% glucose. In contrast, the enzyme production in a solid state fermentation system was 19,550 units/ml extract even when the medium contained 15% glucose. The peak in enzyme titre was, however, shifted from 48 to 72 h. The ability of the solid state fermentation system to significantly overcome catabolic repression was not known earlier and is probably conferred by various physico-chemical factors and culture conditions specific to the system.
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References
Baig MA, Pazlarova J, Vortruba J (1984) Kinetics of α-amylase production in a batch and fed-batch culture of Bacillus subtilis. Folia Microbiol 29:359–364
Bernfeld P (1955) Amylases αβ. Methods Enzymol 1:149–154
D'Appolonia BL, Gilles KA, Osman EM, Pomeranz Y (1971) Carbohydrates. In: Pomeranz Y (ed) Wheat chemistry and technology (Monograph series) vol 3. American Association of Cereal Chemists, St. Paul, Minn., pp 301–329
Emanuilova EI, Toda K (1984) α-Amylase production in batch and continuous culture by Bacillus caldolyticus. Appl Microbiol Biotechnol 19:301–305
Kumar PKR, Lonsane BK (1987) Extraction of gibberellic acid from dry mouldy bran produced under solid state fermentation. Process Biochem 22:139–143
Lonsane BK, Ramesh MV (1990) Production of bacterial thermostable α-amylase by solid-state fermentation: a potential tool for achieving economy in enzyme production and starch hydrolysis. Adv Appl Microbiol 35:1–56
Lonsane BK, Shankaranand VS (1991) Comparative economics of submerged and solid state fermentations. In: The XXXI Annual Conference of the Association of Microbiologists of India, Coimbatore, 23–25 January 1991. AMI Unit, Coimbatore, India, pp 25–32
Meers JL (1972) The regulation of α-amylase production in Bacillus licheniformis. Antonie van Leeuwenhoek J Microbiol Serol 38:585–590
Priest FG, Thirunavukkarasu M (1985) Synthesis and localization of α-amylase and α-glucosidase in Bacillus licheniformis grown in batch and continuous culture. J Appl Bacteriol 58:381–390
Ramesh MV (1989) Production of heat stable bacterial alpha-amylase. Ph.D. Thesis, University of Mysore, Mysore, India
Ramesh MV, Lonsane BK (1987) Solid-state fermentation for production of α-amylase by Bacillus megaterium 16M. Biotechnol Lett 9:323–328
Ramesh MV, Lonsane BK (1989) Solid state fermentation for production of higher titres of thermostable alpha-amylase with two peaks for pH optima by Bacillus licheniformis M27. Biotechnol Lett 11:649–652
Ramesh MV, Lonsane BK (1990) Critical importance of moisture content in alpha-amylase production by Bacillus licheniformis M27 in solid state fermentation. Appl Microbiol Biotechnol 33:501–505
Saito M, Yamamoto K (1975) Regulatory factors affecting α-amylase production in Bacillus licheniformis. J Bacteriol 121:848–856
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Ramesh, M.V., Lonsane, B.K. Ability of a solid state fermentation technique to significantly minimize catabolic repression of α-amylase production by Bacillus licheniformis M27. Appl Microbiol Biotechnol 35, 591–593 (1991). https://doi.org/10.1007/BF00169621
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DOI: https://doi.org/10.1007/BF00169621