Abstract
Experiments were carried out in the isolated spleen of the rat to study in a lymphoid organ the influence of interleukins (ILs) on noradrenaline release. Spleens were perfused with Tyrode's solution and the overflow of endogenous noradrenaline was determined by HPLC with electrochemical detection.
Perivascularelectrical stimulation (4 or 10 Hz, 20–28 mA, 2 min) caused an increase in noradrenaline overflow and in perfusion pressure, both of which were markedly reduced by perfusion with Ca2+-free solution, abolished by tetrodotoxin, unaffected by hexamethonium, and subject to α2-adrenoceptor- and muscarinic receptor-mediated modulation as shown by the effects of rauwolscine and methacholine. Human recombinant IL-1ß and IL-2 and mouse recombinant IL-2 10ng/ml failed to affect the evoked overflow of noradrenaline after an exposure time of 15 min. In contrast, human recombinant IL-1ß and IL-2 0.1 ng/ml reduced the evoked overflow after exposure for 80 min; the inhibition tended to increase 30 min later despite washout. Murine recombinant IL-2 1.2 ng/ml caused no change after contact with the tissue for 80 min but there was an inhibition 30 min later after washout. Human recombinant IL-6 (0.1 ng/ml) caused no significant change.
The inhibitory effect of low concentrations of IL-1ß and IL-2 supports the idea that locally produced mediators of the immune system may affect neuronal function.
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Correspondence to: I. T. Bognar at the above address
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Bognar, I.T., Albrecht, S.A., Farasaty, M. et al. Effects of human recombinant interleukins on stimulation-evoked noradrenaline overflow from the rat perfused spleen. Naunyn-Schmiedeberg's Arch Pharmacol 349, 497–502 (1994). https://doi.org/10.1007/BF00169139
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DOI: https://doi.org/10.1007/BF00169139