Summary
(1) Effects of bepridil (0.3–100 μmol/l) on transmembrane currents which are active during the repolarization of the cardiac action potential were studied in sheep cardiac Purkinje fibres with the two-microelectrode voltage-clamp technique. Transmembrane currents were activated at a frequency of 0.03 Hz. (2) The initial inwardly rectifying current (i K1) was reduced by 1.8 μmol/l bepridil to 70% of the reference i K1-current in the absence of the drug. (3) An initial outward current, which is activated at positive membrane potentials (i inst) was depressed to 70% of reference by 14 μmol/l bepridil. (4) The time-dependent outward current (i K) was decreased by 1.8 μmol/l bepridil to 70% of its amplitude in the absence of bepridil. The biexponential time course of i K-current activation changed to be monoexponential with 100 μmol/l bepridil. The effect of bepridil on i K-current resulted in a shift of the activation curve of i K-current to more positive membrane potentials (10 μmol/l bepridil) and an additional decrease of driving force and/or conductance of the i K-channels with higher bepridil concentrations (100 μmol/l). (5) The transient outward current (i to) was completely blocked by 30 μmol/l bepridil. Inhibition to 70% of reference occurred with 1 μmol/l bepridil. The voltage-dependent inactivation of i to-current was affected by bepridil: the amplitude of the steady-state inactivation curve was reduced and i to-current was inactivated faster after application of bepridil. Bepridil caused no pronounced shift of the steady-state inactivation curve along the voltage axis. (6) The pacemaker current (i f) was slightly increased under the influence of low bepridil concentrations (0.3, 1 μmol/l) while it was reduced to 70% of reference by 100 μmol/l bepridil. (7) The blocking action of bepridil on outward currents in sheep cardiac Purkinje fibres will explain the action potential prolongation, which is observed in different mammalian cardiac tissues under the influence of bepridil.
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References
Anno T, Furuta T, Itho M, Kodama I, Tojama J, Yamada K (1984) Effects of bepridil on the electrophysiological properties of guinea-pig ventricular muscles. Br J Pharmacol 81:589–597
Aronson RS, Gelles JM, Hoffman BF (1973) A new method for producing short cardiac Purkinje fibers suitable for voltage clamp. J Appl Physio1 34:527–530
Baumgarten CM, Isenberg G (1977) Depletion and accumulation of potassium in the extracellular clefts of cardiac Purkinje fibres during voltage clamp hyperpolarization and depolarization. Pflügers Arch 368:19–31
Beaughard M, Ferrier M, Labrid C, Lamar JC, Leboeuf J, Piris P (1982) Studies on the bradycardia induced by bepridil. Br J Pharmacol 75:293–300
Benet LZ (1985) Pharmacokinetics and metabolism of bepridil. Am J Cardiol 55:8C-13C
Borchard U, Berger F, Hafner D (1987a) Effects of the slow channel inhibitor Bepridil on ionic currents in sheep cardiac Purkinje fibres. Naunyn-Schmiedeberg's Arch Pharmacol 335 (Suppl): R54
Borchard UHJ, Berger FA, Hafner DJ (1987b) Effects of the slow channel inhibitor Bepridil on repolarizing and pacemaker currents in sheep cardiac Purkinje fibres. Circulation 76 (Suppl IV):473
Boyett MR, Coray A, McGuigan JAS (1980) Cow ventricular muscle. I. The effect of the extracellular potassium concentration on the current-voltage relationship. II. Evidence for a time-dependent outward current. Pflügers Arch 389:37–44
Chollet-Debord F, Moins N, Gachon P, Duchene-Marullaz P (1986) Comparison of verapamil and bepridil, two slow channel inhibitors, in protection against calcium-induced arryhthmias. Naunyn-Schmiedeberg's Arch Pharmacol 334:105–109
Coraboeuf E, Carmeliet E (1982) Existence of two transient outward currents in sheep cardiac Purkinje fibers. Pflügers Arch 392:352–359
Dangman KH (1985) Effects of bepridil on transmembrane action potentials recorded from isolated canine cardiac tissues. Naunyn-Schmiedeberg's Arch Pharmacol 329:326–332
Deck KA, Kern R, Trautwein W (1964) Voltage clamp technique in mammalian cardiac fibres. Pflügers Arch 280:50–62
DiBianco R, Alpert J, Katz RJ, Spann J, Chesler E, Ferri DP, Larca LJ, Costello RB, Gore JM, Eisenman MJ (1984) Bepridil for chronic stable angina pectoris: results of a prospective multicenter placebo-controlled dose-ranging study in 77 patients. Am J Cardiol 53:35
Dietrich H, Borchard U, Hafner D, Hirth C (1985) Antiarrhythmic and electrophysiological actions of flecainide, bepridil and amiodarone on isolated heart preparations during controlled hypoxia. Arch Int Pharmacodyn Ther 274:267–282
DiFrancesco D (1982) Block and activation of the pace-maker channel in calf Purkinje fibres: effects of potassium, caesium and rubidium. J Physiol (Lond) 329:485–507
DiFrancesco D, McNaughton PA (1979) The effects of calcium on outward membrane currents in the cardiac Purkinje fibre. J Physiol (Lond) 289:347–373
DiFrancesco D, Noble D (1985) A model of cardiac electrical activity incorporating ionic pumps and concentration changes. Philos Trans R Soc Lond (Biol) 307:353–398
DiFrancesco D, Ferroni A, Visentin S (1984) Barium-induced blockade of the inward rectifier in calf Purkinje fibres. Pflügers Arch 402:446–453
Duchêne-Marullaz P, Kantelip J-P, Trolèse J-F (1983) Effects of bepridil, a new antianginal agent, on ambulatory electrocardiography in human volunteers. J Cardiovasc Pharmacol 5:506–510
Fozzard HA, Hiraoka M (1973) The positive dynamic current and its inactivation properties in cardiac Purkinje fibres. J Physiol (Lond) 234:569–586
Godfraind T (1987) Classification of calcium antagonists. Am J Cardiol 59:11B-23B
Hill JA, O'Brien JT, Scott E, Conti CR, Pepine CJ (1984) Effects of bepridil on exercise tolerance in chronic stable angina: a double-blind randomized, placebo-controlled crossover trial. Am J Cardiol 53:679
Isenberg G (1977a) Cardiac Purkinje fibres. (Ca+)i controls steady state potassium conductance. Pflügers Arch 371:71–76
Isenberg G (1977b) Cardiac Purkinje fibres. (Ca2+)i controls the potassium permeability via the conductance components gK1 and gK2. Pflügers Arch 371:77–85
Isenberg G, Vereecke J, van der Heyden G, Carmeliet E (1983) The shortening of the action potential by DNP in guinea-pig ventricular myocytes is mediated by an increase of a time-independent K conductance. Pflügers Arch 397:251–259
Kane KA, Winslow E (1980) Antidysrhythmic and electrophysiological effects of a new antianginal agent, bepridil. J Cardiovasc Pharmacol 2:193–203
Kane KA, Berdeja Garcia GY, Sánchez-Pérez S, Pastelin G (1983) Electrophysiological effects of lidocaine, 1-chlorpheniramine, and bepridil on rabbit sinus node pacemaker cells. J Cardiovasc Pharmacol 5:102–108
Kato R, Singh BN (1986) Effects of bepridil on the electrophysiologic properties of isolated canine and rabbit myocardial fibers. Am Heart J 111:271–279
Labrid C, Grosset A, Dureng G, Mironneau J, Duchene-Marullaz P (1979) Some membrane interactions with bepridil, a new antianginal agent. J Pharmacol Exp Ther 211:546–554
Labrid C, Leinot M, Beaughard M, Basiez M, Duchene-Marullaz P (1981) Comparative antidysrhythmic profiles of bepridil, amiodarone and disopyramide in the guinea-pig and dog. Arch Int Pharmacodyn Ther 249:87–97
Marshall RJ, Muir AW (1981) The beneficial actions of bepridil in acute myocardial infarction in anaesthetized dogs. Br J Pharmacol 73:471–479
McDonald TF, Trautwein W (1978) The potassium current underlying delayed rectification in cat ventricular muscle. J Physiol (Lond) 274:217–246
Narahara KA, Shapiro W, Weliky I, Park J (1985) Evaluation of bepridil, a new antianginal agent. Clinical and hemodynamic alterations during the treatment of stable angina pectoris. Am J Cardiol 53:29
Noble D, Tsien RW (1968) The kinetics and rectifier properties of the slow potassium current in cardiac Purkinje fibres. J Physiol (Lond) 195:185–214
Noble D, Tsien RW (1969) Outward membrane currents activated in the plateau range of potentials in cardiac Purkinje fibres. J Physiol (Lond) 200:205–231
Sakmann B, Trube G (1984) Voltage-dependent inactivation of inward-rectifying single-channel currents in the guinea-pig heart cell membrane. J Physiol (Lond) 347:659–683
Schneider W, Mehlhorn C, Kaltenbach M, Bussmann W-D (1985) Die antianginöse Wirksamkeit des Calciumantagonisten Bepridil bei der stabilen Angina pectoris. Z Kardiol 74:341
Singh BN, Nademanee K, Feld G, Piontek M, Schwab M (1985) Comparative electrophysiologic profiles of calcium antagonists with particular reference to bepridil. Am J Cardiol 55:14C-19C
Somberg J, Torres V, Flowers D, Miura D, Butler B, Gottlieb S (1985) Prolongation of QT interval and antiarrhythmic action of bepridil. Am Heart J 109:19–27
Vaughan Williams EM (1975) Classification of antidysrhythmic drugs. Pharmacol Ther B 1:115–138
Vogel S, Crampton R, Sperelakis N (1979) Blockade of myocardial slow channels by bepridil (CERM-1978). J Pharmacol Exp Ther 210:378–385
Winslow E, Kane KA (1981) Supraventricular antidysrhythmic and electrophysiological effects of bepridil, a new antianginal agent. J Cardiovasc Pharmacol 3:655–667
Yatani A, Brown AM, Schwartz A (1986) Bepridil block of cardiac calcium and sodium channels. J Pharmacol Exp Ther 237:9–17
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Supported by the Deutsche Forschungsgemeinschaft SFB 242, C 1
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Berger, F., Borchard, U. & Hafner, D. Effects of the calcium entry blocker bepridil on repolarizing and pacemaker currents in sheep cardiac Purkinje fibres. Naunyn-Schmiedeberg's Arch Pharmacol 339, 638–646 (1989). https://doi.org/10.1007/BF00168656
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DOI: https://doi.org/10.1007/BF00168656