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Modulation of noradrenaline and neuropeptide Y (NPY) release in the pig kidney in vivo: involvement of alpha2, NPY and angiotensin II receptors

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Summary

The modulation of the release of noradrenaline (NA) and neuropeptide Y-like immunoreactivity (NPY-LI) was investigated in the pig kidney in vivo. Under control conditions a reproducible co-release of NA and NPY-LI was obtained upon stimulation of the renal nerves with 5 Hz for 1 min. Infusion of peptide YY (PYY, 1 μg/kg/min i.v.), which binds to NPY receptors, caused renal vasoconstriction and reduced the stimulation-evoked overflow of NA and NPY-LI by 24 ± 4 and 33 ± 11%, respectively (P < 0.01). The PYY effect was reversible and was absent 1 h after the infusion. The alpha2-adrenoceptor antagonist yohimbine (0.2 mg/kg i. v.) enhanced the overflow of NA and NPY-LI 2- to 3-fold. The angiotensin converting enzyme inhibitor captopril (5 mg/kg i. v.) did not significantly affect the overflow of NA or NPY-LI evoked by the nerve stimulation. Angiotensin II (0.5 μg/kg/min i. v.), on the other hand, induced a reversible enhancement of the overflow of both NA and NPY-LI by 71 and 77%, respectively (P<0.01). Infusion of endothelin (0.2 μg/kg/min i.v.), which reduced renal blood flow by a magnitude similar to that evoked by angiotensin II, did not significantly alter the nerve stimulation-evoked overflow of NA or NPY-LI. None of the administered drugs did significantly affect the percentage reduction in renal blood flow evoked by nerve stimulation.

It is concluded that the release of NA and NPY-LI from sympathetic nerves in the pig kidney is inhibited in parallel via activation of NPY receptors by PYY and via alpha2-adrenoceptors by endogenous NA. Furthermore, angiotensin II receptor stimulation facilitates the release whereas endothelin has no major effect on transmitter secretion.

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References

  • Bradley T, Hjemdahl P (1986) Renal extraction of endogenous and radiolabelled catecholamines in the dog. Acta Physiol Scand 126:505–510.

    Google Scholar 

  • Dahlöf C, Dahlöf P, Lundberg JM (1985) Neuropeptide Y (NPY) reduces field stimulation-evoked release of noradrenaline and enhances force of contraction in the rat portal vein. Naunyn-Schmiedeberg's Arch Pharmacol 328:327–330.

    Google Scholar 

  • De Jonge A, Knape JTA, van Meel JC, Kalkman HO, Wilffert B, Thoolen MJMC, Timmermanns PBMWM, van Zwieten PA (1982) Effect of converting enzyme inhibition and angiotensin receptor blockade on the vasoconstriction mediated by α1- and α2-adrenoceptor stimulation in pithed normotensive rats. Naunyn-Schmiedeberg's Arch PharmacoL 321:309–313.

    Google Scholar 

  • Ekblad E, Edvinsson L, Wahlestedt C, Uddman R, Håkanson R, Sundler F (1984) Neuropeptide Y co-exists and co-operates with noradrenaline in perivascular nerve fibers. Regul Peptides 8:225–235.

    Google Scholar 

  • Franca-Cereceda A, Lundberg JM, Dahlöf C (1985) Neuropeptide Y and sympathetic control of heart contractility and coronary vascular tone. Acta Physiol Scand 124:361–369.

    Google Scholar 

  • Fried G, Lundberg JM, Theodorsson-Norheim E (1985) Subcellular storage and axonal transport of neuropeptide Y (NPY) in relation to catecholamines in the cat. Acta Physiol Scand 125:145–154.

    Google Scholar 

  • Hjemdahl P (1987) Catecholamine measurement in plasma by high-performance liquid chromatography with electrochemical detection. Methods Enzymol 142:521–534.

    Google Scholar 

  • Kopp U, Bradley T, Hjemdahl P (1983) Renal venous outflow and urinary excretion of norepinephrine, epinephrine and dopamine during graded renal nerve stimulation. Am J Physiol 244:E52-E60.

    Google Scholar 

  • Langer SZ (1981) Presynaptic regulation of the release of catecholamines. Pharmacol Rev 32:337–362.

    Google Scholar 

  • Lundberg JM, Stjärne L (1984) Neuropeptide Y (NPY) depresses the secretion of 3H-noradrenaline and the contractile response evoked by field stimulation, in the rat vas deferens. Acta Physiol Scand 120:477–479.

    Google Scholar 

  • Lundberg JM, Tatemoto K (1982) Pancreatic polypeptide family (APP, BPP, NPY and PYY) in relation to sympathetic vasoconstriction resistant to alpha-adrenoceptor blockade. Acta Physiol Scand 116:393–402.

    Google Scholar 

  • Lundberg JM, Tatemoto K, Terenius L, Hellström PM, Mutt V, Hökfelt T, Hamberger B (1982a) Localization of peptide YY (PYY) in gastrointestinal endocrine cells and effects on intestinal blood flow and motility. Proc Natl Acad Sci [USA] 79:4471–4475.

    Google Scholar 

  • Lundberg JM, Terenius L, Hökfelt T, Martling C-R, Tatemoto K, Mutt V, Polak J, Bloom SR, Goldstein M (1982b) Neuropeptide Y (NPY)-like immunoreactivity in peripheral noradrenergic neurons and effects of NPY on sympathetic function. Acta Physiol Scand 116:477–480.

    Google Scholar 

  • Lundberg JM, Terenius L, Hökfelt T, Goldstein M (1983) High levels of neuropeptide Y in peripheral noradrenergic neurons in various mammals including man. Neurosci Lett 42:167–172.

    Google Scholar 

  • Lundberg JM, Terenius L, Hökfelt T, Tatemoto K (1984) Comparative immunohistochemical and biochemical analysis of pancreatic polypeptide-like peptides with special reference to presence of neuropeptide Y in central and peripheral neurons. J Neurosci 4:2376–2386.

    Google Scholar 

  • Lundberg JM, Pernow J, Franco-Cereceda A, Rudehill A (1987) Effects of antihypertensive drugs on sympathetic vascular control in relation to neuropeptide Y. J Cardiovasc Pharmacol 10 (Suppl 12):S51-S68.

    Google Scholar 

  • Lundberg JM, Hemsén A, Larsson O, Rudehill A, Saria A, Fredholm BB (1988) Neuropeptide Y receptor in pig spleen: binding characteristics, reduction in cyclic AMP formation and calcium antagonist inhibition of vasoconstriction. Eur J Pharmacol 145:21–29.

    Google Scholar 

  • Majewski H, Hedler L, Schurr C, Starke K (1984) Modulation of noradrenaline release in the pithed rabbit: a role for angiotensin II. J Cardiovasc Pharmacol 6:888–896.

    Google Scholar 

  • Mauss U, Gerstberger R, Gimpl G, Lang RE (1988) Evidence for NPY binding sites in the rabbit kidney. Naunyn-Schmiedeberg's Arch Pharmacol 337 (Suppl):R108.

    Google Scholar 

  • Mohy El-Din MM, Malik KU (1988) Neuropeptide Y stimulates renal prostaglandin synthesis in the isolated rat kidney: contribution of Ca2+ and calmodulin. J Pharmacol Exp Ther 246: 479–484.

    Google Scholar 

  • Pernow J (1988) Co-release and functional interactions of neuropeptide Y and noradrenaline in peripheral sympathetic vascular control. Acta Physiol Scand 133 (Suppl 568):1–56.

    Google Scholar 

  • Pernow J, Lundberg JM (1989) Release and vascoconstrictor effects of neuropeptide Y in relation to non-adrenergic sympathetic control of renal blood flow in the pig. Acta Physiol Scand 136:507–518.

    Google Scholar 

  • Pernow J, Saria A, Lundberg JM (1986) Mechanisms underlying pre- and postjunctional effects of neuropeptide Y in sympathetic vascular control. Acta Physiol Scand 126:239–249.

    Google Scholar 

  • Pernow J, Kahan T, Hjemdahl P, Lundberg JM (1988a) Possible involvement of neuropeptide Y in sympathetic control of canine skeletal muscle. Acta Physiol Scand 132:43–50.

    Google Scholar 

  • Pernow J, Lundberg JM, Kaijser L (1988b) α-Adrenoceptor influence on plasma levels of neuropeptide Y-like immunoreactivity and catecholamines during rest and sympathoadrenal activation in humans. J Cardiovasc Pharmacol 12:593–599.

    Google Scholar 

  • Robie NW (1980) Evaluation of presynaptic α-receptor function in the canine renal vascular bed. Am J Physiol 239:H422-H426.

    Google Scholar 

  • Starke K (1977) Regulation of noradrenaline release by presynaptic receptor systems. Rev Physiol Biochem Pharmacol 77:1–124.

    Google Scholar 

  • Starke K (1987) Presynaptic α-autoreceptors. Rev Physiol Biochem Pharmacol 107:73–146.

    Google Scholar 

  • Tatemoto K (1982) Neuropeptide Y: complete amino acid sequence of the brain peptide. Proc Natl Acad Sci [USA] 79:5485–5489.

    Google Scholar 

  • Tatemoto K, Carlquist M, Mutt V (1982) Neuropeptide Y — a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature 296:659–660.

    Google Scholar 

  • Theodorsson-Norheim E (1987) Friedman and Quade test: BASIC computer program to perform non-parametric one-way analysis of variance and multiple comparisons on rank of several related samples. Comput Biol Med 17:85–99.

    Google Scholar 

  • Theodorsson-Norheim E, Hemsén A, Lundberg JM (1985) Radioimmunoassay for neuropeptide Y (NPY): chromatographic characterization of immunoreactivity in plasma and tissue extracts. Scand J Clin Lab Invest 45:355–365.

    Google Scholar 

  • Westfall TC (1977) Local regulation of adrenergic neurotransmission. Physiol Rev 57:659–728.

    Google Scholar 

  • Wiklund NP, Öhlén A, Cederqvist B (1988) Inhibition of adrenergic neuroeffector transmission by endothelin in the guinea-pig femoral artery. Acta Physiol Scand 134:311–312.

    Google Scholar 

  • Williams GH (1988) Converting-enzyme inhibitors in the treatment of hypertension. New Eng J Med 319:1517–1525.

    Google Scholar 

  • Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, Yazaki Y, Goto K, Masaki T (1988) A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332:411–415.

    Google Scholar 

  • Zimmerman BG (1978) Actions of angiotensin on adrenergic nerve endings. Federation Proc 37:199–202.

    Google Scholar 

  • Zimmerman BG, Liao JC, Gisslen J (1971) Effect of phenoxybenzamine and combined administration of iproniazid and tropolone on catecholamine release elicited by renal sympathetic nerve stimulation. J Pharmacol Exp Ther 176:603–610.

    Google Scholar 

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  1. Department of Pharmacology, Karolinska Institutet, Box 60400, S-10401, Stockholm, Sweden

    John Pernow & Jan M. Lundberg

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  1. John Pernow
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  2. Jan M. Lundberg
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Pernow, J., Lundberg, J.M. Modulation of noradrenaline and neuropeptide Y (NPY) release in the pig kidney in vivo: involvement of alpha2, NPY and angiotensin II receptors. Naunyn-Schmiedeberg's Arch Pharmacol 340, 379–385 (1989). https://doi.org/10.1007/BF00167038

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  • DOI: https://doi.org/10.1007/BF00167038

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