Summary
The aim of the present work was to demonstrate the presence of presynaptic β-adrenoceptors in the rabbit isolated pulmonary artery by investigating the effect of isoprenaline on 3H-noradrenaline (3H-NA) release evoked by electrical field stimulation. (−)-Isoprenaline (10−7–10−6 mol/1) had no effect on the 3H-overflow evoked by stimulation (3 Hz) of the pulmonary artery preloaded with 3H-NA. At 10−5 mol/1, (−)-isoprenaline reduced the 3-Hoverflow by maximally 39%. (−)-Isoprenaline (10− mol/1) caused an inhibition that remained almost constant with time. The same results were obtained with (−)-isoprenaline (10−7 − 3 × 10−5 mol/1) in the presence of cocaine (3 × 10−5 mol/1), corticosterone (4 × 10−5 mol/1), and the catechol-O-methyltransferase inhibitor U-0521 (3′-4′-dihydroxy-2-methylpropiophenone) (10−4 mol/1). In the presence of cocaine plus corticosterone, (−)-isoprenaline (3 × 10−10–10−7 mol/1) had no effect on the 3H-overflow evoked by stimulation at 1 Hz. At 10−6 mol/1, (−)-isoprenaline slightly reduced the 3H-overflow. At 10 Hz, (−)-isoprenaline (10−6−3 × 10−5 mol/1) decreased the 3-Hoverflow and had no effect at 10−7 mol/1. In the presence of either rauwolscine (10−6 mol/1), phentolamine (10−6 mol/1) or the phosphodiesterase inhibitor ICI 63,197 (3 × 10−5 mol/1), (−)-isoprenaline (10−7 – 10−6 mol/1) did not enhance the stimulation-evoked 3H-overflow. (±)-Pro-pranolol (10−7–10−5 mol/1) did not alter the stimulationevoked 3H-overflow. At 3 × 10−5 mol/1, this antagonist reduced the neurogenic response by 50%. (±)-Propranolol (10−6 mol/1) did not alter the effect of (−)-isoprenaline (10−7-3 × 10−5 mol/1) on the stimulation-evoked 3H-overflow. (+)-Isoprenaline (10−7 − 3 × 10−5 mol/1) enhanced slightly the stimulation-evoked 3H-overflow. No enhancement was seen in the presence of either (±)-propranolol (10−6 mol/1) or rauwolscine(10−6 mot/1). The results suggest that postganglionic sympathetic nerve terminals in rabbit pulmonary artery do not possess facilitatory β-adrenoceptors.
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Nedergaard, O.A. Effect of isoprenaline on noradrenaline release from sympathetic neurones in rabbit isolated pulmonary artery. Naunyn-Schmiedeberg's Arch Pharmacol 336, 176–182 (1987). https://doi.org/10.1007/BF00165802
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DOI: https://doi.org/10.1007/BF00165802