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Effect of donor cornea preservation in tissue culture and in McCarey-Kaufmann medium on corneal graft rejection and visual acuity

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Abstract

In a retrospective study the rate of rejection and clouding of the donor cornea for other reasons was investigated in 230 penetrating keratoplasties performed between 1984–1986. Donor corneas were stored in McCarey-Kaufmann Medium (MK) at 4 °C or in a modified Minimal Essential Medium (MEM) at 31 °C. No statistical differences in rejection rate, cloudiness due to other causes or visual acuity was found between MEM- and MK-stored donor corneas.

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References

  1. Forster RK, Fine M. Relation of donor age to success in penetrating keratoplasty. Arch Ophthalmol 1971; 85: 42–47.

    Google Scholar 

  2. McCarey BE, Kaufmann HE. Improved corneal storage. Invest Ophthalmol 1974; 13: 165–173.

    Google Scholar 

  3. Sperling S, Olsen T, Ehlers N. Fresh and cultured grafts compared by post-operative thickness and endothelial cell density. Acta Ophthalmol 1981; 59: 566–575.

    Google Scholar 

  4. Pels E, Schuchard Y. Organ-culture preservation of human cornea. Doc Ophthalmol 1983; 56: 147–153.

    Google Scholar 

  5. Andersen J, Ehlers N. Corneal transplantation using long-term cultured donor material. Acta Ophthalmol 1986; 64: 93–96.

    Google Scholar 

  6. Beekhuis WM, van Rij G, Zivojnovic R. Silicone oil keratopathy: Indications for keratoplasty. Br J Ophthalmol 1985; 69: 247–253.

    Google Scholar 

  7. Pels E, Schuchard Y. Organ culture and endothelial evaluation as a preservation method for human cornea. In: Brightbill FS, ed. Corneal surgery. St Louis: Mosby, 1986: 93–102.

    Google Scholar 

  8. Van Rij G, de Ruiter W, Vijfvinkel G. Corneal punch block for disposable superblade trephine. Am J Ophthalmol 1983; 95: 564–565.

    Google Scholar 

  9. Khodadoust AA. Corneal graft failure. In: Ciba Foundation Symposium. Associated Scientific Publisher, 1973: 344.

  10. Ehlers N, Sperling S, Olsen T. Post-operative thickness and endothelial cell density in cultivated, cryopreserved human corneal grafts. Acta Ophthalmol 1982; 60: 935–944.

    Google Scholar 

  11. Völker-Dieben HJ, d'Amaro J, Kok-van Alphen CC, Pels E. The survival of organ cultured donor corneas. In: Brightbill FS ed. Corneal surgery. St Louis: Mosby, 1986: 102–113.

    Google Scholar 

  12. de Beijer-Dominicus JA, van Rij G, Beekhuis WH, Renardel de Lavalette JGC, Pels E, Rinkel-van Driel B. The cornea. Transactions of the World Congress of the Cornea III. 1988; 20: 115–116.

    Google Scholar 

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Authors and Affiliations

  1. Rotterdam Eye Hospital, Erasmus University, Rotterdam, The Netherlands

    A. C. Moll, W. H. Beekhuis, J. H. C. Reneradel De Lavalette & E. Rinkel-Van Driel

  2. Dept. of Ophthalmology, University Hospital, Groningen, The Netherlands

    G. Van Rij

  3. Dept. of Medical Statistics, University, Leiden

    J. Hermans

  4. Interuniversitair Oogheelkundig Instituut, Amsterdam, The Netherlands

    E. Pels

Authors
  1. A. C. Moll
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  2. G. Van Rij
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  3. W. H. Beekhuis
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  4. J. H. C. Reneradel De Lavalette
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  5. J. Hermans
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  6. E. Pels
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  7. E. Rinkel-Van Driel
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Moll, A.C., Van Rij, G., Beekhuis, W.H. et al. Effect of donor cornea preservation in tissue culture and in McCarey-Kaufmann medium on corneal graft rejection and visual acuity. Doc Ophthalmol 78, 273–278 (1991). https://doi.org/10.1007/BF00165691

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  • DOI: https://doi.org/10.1007/BF00165691

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