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Pharmacological characterisation of neurokinin receptors mediating anion secretion in rat descending colon mucosa

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Summary

Substance P(SP), neurokinin A (NKA), neurokinin B (NKB), [Sar9, Met (O2)11]-SP (SMSP), senktide, [βAla8]-NKA(4–10) and neuropeptide γ (NPγ) all stimulate secretory responses in rat descending colon mucosa under voltage clamp conditions. Secretory responses (measured as short circuit current under voltage clamp conditions) were transient and those evoked by SP, SMSP, NKA and senktide were significantly reduced by pretreating tissues with the chloride channel blocker, diphenylamine carboxylate (DPC). Concentration-response curves showed varying degrees of sensitivity to tetrodotoxin (TTX). Senktide-induced secretion was virtually abolished by TTX, while NPγ and [βAla8]-NKA(4–10) were not significantly altered. Rightward shifts of concentration-response curves were observed for SMSP, NKA and SP in TTX treated preparations compared with controls. NKA response curves in the presence of TTX were further inhibited by MEN10,207 and CP-96,345. GR71251, GR82334 and CP-96,345 all inhibited SMSP secretory responses with pA2 values of 5.8, 6.5 and 6.9 respectively. In conclusion three types of neurokinin receptor exist in preparations of rat colon mucosa and their relative location within neuronal and epithelial surfaces are discussed.

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Authors and Affiliations

  1. Department of Pharmacology, The Royal College of Surgeons of England, 35-43 Lincoln's Inn Fields, WC2A 3PN, London, UK

    Helen M. Cox, Iain R. Tough, Kathryn Grayson & Simon Yarrow

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  1. Helen M. Cox
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  2. Iain R. Tough
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  3. Kathryn Grayson
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  4. Simon Yarrow
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Additional information

Correspondence to H. M. Cox at the above address

K. G. and S. Y. were project students with H. M. C. in the Department of Pharmacology, University of Cambridge. S. Y. was also a Wellcome Trust Vacation Scholar

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Cox, H.M., Tough, I.R., Grayson, K. et al. Pharmacological characterisation of neurokinin receptors mediating anion secretion in rat descending colon mucosa. Naunyn-Schmiedeberg's Arch Pharmacol 348, 172–177 (1993). https://doi.org/10.1007/BF00164795

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  • DOI: https://doi.org/10.1007/BF00164795

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