Skip to main content
SpringerLink
Log in

Myeloma affects both the growth and function of human osteoblast-like cells

  • Published:
Clinical & Experimental Metastasis Aims and scope Submit manuscript

Abstract

Myeloma behaves differently to other osteolytic tumours which metastasize to bone, in that the latter usually provoke reactive bone formation in the host bone. A previous study showed that a myeloma cell line (GM1500) secreted an osteoblast-inhibiting factor(s). The present study was undertaken to determine whether other myeloma cells also secreted a factor(s) which inhibited both cell proliferation and DNA synthesis of osteoblast-like cells and whether the myeloma also affected the function of osteoblasts. The results showed that a second cell line (Karpas 707) as well as myeloma tissue taken from two patients had a similar effect. The myeloma cells did not affect total collagen or protein synthesis, and did not affect the overall degree of mineralization. A biphasic effect was seen on alkaline phosphatase activity. Thus, although the proliferation of the pre-osteoblast was affected, the synthetic functions of the osteoblasts were not.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Galasko CSB, 1986, Skeletal Metastases (London: Butterworths).

    Google Scholar 

  2. Galasko CSB, 1975, The pathological basis for skeletal scintigraphy. Journal of Bone and Joint Surgery, 57B, 353–359.

    Google Scholar 

  3. Milch RA and Changus GW, 1956, Response of bone to tumour invasion. Cancer, 9, 340–351.

    Article  PubMed  CAS  Google Scholar 

  4. Galasko CSB, 1982, Mechanism of lytic and blastic metastatic disease of bone. Clinical Orthopaedics Related Research, 169, 269–285.

    Google Scholar 

  5. Evans CE, Galasko CSB and Ward C, 1989, Does myeloma secrete an osteoblast inhibiting factor? Journal of Bone and Joint Surgery, 71B, 288.

    Google Scholar 

  6. Gallagher JA, Beresford JN, Sharrad M, Gowen M, Poser J, MacDonald E, McGuire MJB and Russell RGG, 1983, Human bone cell in culture—a novel system for the investigation of osteoblast function. Calcified Tissue International, 35 (Suppl), A24.

    Google Scholar 

  7. Ashton BA, Abdullah F, Cave J, Williamson M, Sykes BC, Couch M and Poser J, 1985, Characterization of cells with high alkaline phosphatase activity derived from human bone marrow: preliminary assessment of their osteogenicity. Bone, 6, 313.

    Article  PubMed  CAS  Google Scholar 

  8. Auf'mkolk B and Schwartz ER, 1985, Biochemical characterizations of human osteoblasts in culture. Normal and Abnormal Bone Growth: basic and clinical research (New York: A. R. Liss), pp. 201–204.

    Google Scholar 

  9. Evans CE, Galasko CSB and Ward C, 1990, Effect of donor age on the growth in vitro of cells obtained from human trabecular bone. Journal of Orthopaedic Research, 8, 234.

    Article  PubMed  CAS  Google Scholar 

  10. Evans CE, Galasko CSB and Ward C, 1986, In vitro proliferation of cells cultured from human cancerous bone. Cell Medicated Calcification and Matrix Vesicles, edited by Ali SY (Amsterdam: Elsevier), pp. 303–306.

    Google Scholar 

  11. Karpas A, Fischer P and Swirsky D, 1982, Human myeloma cell line carrying a Philadelpia chromosome. Science, 216, 997–999.

    Article  PubMed  CAS  Google Scholar 

  12. Durie BGM, Salmon SE and Murphy GR, 1981, Relation of osteoclast activating factor to extent of bone disease in multiple myeloma. British Journal of Haematology, 47, 21–30.

    PubMed  CAS  Google Scholar 

  13. Lowry OH, 1955, A sensitive method for assay of phosphatases using dinitrophenol. Methods in Enzymology, 4, 371–372.

    Google Scholar 

  14. Ackerman GA, 1962, Substituted naphthol AS phosphate derivatives for the localization of leucocyte alkaline phosphatase activity. Laboratory Investigation, 11, 563.

    PubMed  CAS  Google Scholar 

  15. Peterkovsky B and Diegelman R, 1971, Use of a proteinase-free collagenase for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry, 10, 988.

    Article  Google Scholar 

  16. Lowry OH, Rosebrough NJ, Farr AL and Randall RJ, 1951, Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265.

    PubMed  CAS  Google Scholar 

  17. Simmons DSJ, Kent GN, Jilka RL, Scott DM, Fallon M and Cohn DV, 1982, Formation of bone by isolated, cultured osteoblasts in millipore filter chambers. Calcified Tissue International, 34, 291.

    Article  PubMed  CAS  Google Scholar 

  18. Bellows CG, Aubin JE, Heersche JNM and Antosz ME, 1986. Mineralized bone nodules formed in vitro, from enzymatically released rat calvaria cell populations. Calcified Tissue International, 38, 143.

    Article  PubMed  CAS  Google Scholar 

  19. Stein GS and Lian JB, 1989, Molecular mechanisms associated with the relationship of cell growth and differentiation in osteoblasts. Proceedings of the American Society for Bone and Mineral Research, Montreal, pp. 16–24.

  20. Owen M, 1980, The origin of bone cells in the post-natal organism. Arthritis and Rheumatism, 23, 1073.

    Article  PubMed  CAS  Google Scholar 

  21. Gerstenfeld LC, Chipman SD, Glowacki J and Lian JB, 1987, Expression of differential function by mineralizing cultures of chicken osteoblasts. Development Biology, 122, 49.

    Article  CAS  Google Scholar 

  22. Shalhoub V, Gerstenfeld LC, Collart D, Lian JB and Stein GS, 1989, Downregulation of cell growth and cell cycle regulated genes during chick osteoblast differentiation with the reciprocal expression of histone variants. Biochemistry, 28, 5318.

    Article  PubMed  CAS  Google Scholar 

  23. Klein B, Jourdan M, Durie BGM, Rossi JF and Bataille R, 1987, Spontaneous production of bone cytokines by human myeloma cell lines. Journal of Bone and Mineral Research, 2, 41.

    Google Scholar 

  24. Mercola M and Stiles CD, 1988, Growth factor superfamilies and mammalian embryogenesis. Development, 102, 451.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. University of Manchester Department of Orthopaedic Surgery, Clinical Sciences Building, Hope Hospital, Eccles Old Road, M6 8HD, Salford, UK

    Carol Elizabeth Evans, Carol Ward, Lakvinder Rathour & Charles Bernard Galasko

Authors
  1. Carol Elizabeth Evans
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Carol Ward
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lakvinder Rathour
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Charles Bernard Galasko
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Evans, C.E., Ward, C., Rathour, L. et al. Myeloma affects both the growth and function of human osteoblast-like cells. Clin Exp Metast 10, 33–38 (1992). https://doi.org/10.1007/BF00163574

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00163574

Keywords

Search

Navigation