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Selection of host cell lines for scale-up growth conditions of a recombinant baculovirus vector expressing the measles virus nucleocapsid protein

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Biotechnology Techniques

Summary

Various insect cell lines were grown as suspension cultures in spinner vessels and infected with a recombinant baculovirus vector expressing the measles virus nucleoprotein. The highest yields of recombinant protein production were achieved using Trichoplusia ni (BTI-Tn 5B1-4) cells growing as natural aggregates in suspension and cell line Mb as a single cell suspension culture.

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Author notes

  1. I Hutchinson

    Present address: University of Greenwich, SE18 6PF, Woolwich, London, UK

  2. R Smith

    Present address: Cantab, Cambridge Science Park, CB4 4GN, Cambridge, UK

Authors and Affiliations

  1. Centre for Applied Microbiology and Research (CAMR), Porton Down, SP4 OJG, Salisbury, Wiltshire, UK

    I Hutchinson, A R Fooks, R Smith & G N Stacey

Authors
  1. I Hutchinson
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  2. A R Fooks
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  3. R Smith
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  4. G N Stacey
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Hutchinson, I., Fooks, A.R., Smith, R. et al. Selection of host cell lines for scale-up growth conditions of a recombinant baculovirus vector expressing the measles virus nucleocapsid protein. Biotechnol Tech 9, 907–912 (1995). https://doi.org/10.1007/BF00158545

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  • DOI: https://doi.org/10.1007/BF00158545

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