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Simple and rapid determination of the activity of recombinant D-amino acid oxidase in cephalosporin C bioconversion with use of a micro pO2 probe

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Biotechnology Techniques

Summary

D-Amino acid oxidase (DAAO) genes were amplified from Trigonopsis variabilis and Rhodotorula gracilis by polymerase chain reactions. T. variabilis DAAO gene was cloned into a pUC19 vector. The expression of the T. variabilis DAAO was directly determined in permeabilized E. coli cells using a micro pO2 probe. The micro pO2 probe was sensitive enough to be suitable for a simple and rapid estimation of DAAO activity toward cephalosporin C.

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Authors and Affiliations

  1. Department of Applied Microbiology, Yeungnam University, 712-749, Kyongsan, Republic of Korea

    In-Wook Kim & Yong-Ho Khang

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  1. In-Wook Kim
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  2. Yong-Ho Khang
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Kim, IW., Khang, YH. Simple and rapid determination of the activity of recombinant D-amino acid oxidase in cephalosporin C bioconversion with use of a micro pO2 probe. Biotechnol Tech 9, 863–868 (1995). https://doi.org/10.1007/BF00158537

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  • DOI: https://doi.org/10.1007/BF00158537

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