Skip to main content
SpringerLink
Log in

Detection of dilute antigen in large sample volumes using antibody-coated polyester cloth

  • Published:
Biotechnology Techniques

Summary

A model antibody, goat anti-rabbit IgG antibody, was adsorbed onto a disk of polyester cloth and then fixed into a column apparatus. The macroporosity of the cloth allowed rapid immunoconcentration of a model antigen, rabbit IgG, by passing a large volume of the dilute antigen through the antibody-coated cloth. Such immunoconcentration permitted detection of the dilute antigen which otherwise would have gone undetected.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Blais, B.W. and Yamazaki, H. (1989a). Biotechn. Techn., 3, 23–26.

    Google Scholar 

  • Blais, B.W. and Yamazaki, H. (1989b). Int. J. Food Microbiol., 9, 63–71.

    Google Scholar 

  • Tijseen, P. (1985). Practice and Theory of Enzyme Immunoassay. pp. 301–305, Amsterdam: Elsevier.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology and Institute of Biochemistry, Carleton University, K1S 5B6, Ottawa, Ontario, Canada

    Scott Boyd & Hiroshi Yamazaki

Authors
  1. Scott Boyd
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hiroshi Yamazaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boyd, S., Yamazaki, H. Detection of dilute antigen in large sample volumes using antibody-coated polyester cloth. Biotechnol Tech 9, 849–852 (1995). https://doi.org/10.1007/BF00158534

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00158534

Keywords

Search

Navigation