Skip to main content
SpringerLink
Log in

Biosensor for the monitoring of hydrogen peroxide using poly(vinyl alcohol) membrane system

  • Published:
Biotechnology Techniques

Summary

A biosensor system for continuous on-line monitoring of hydrogen peroxide concentration was developed employing catalase and a poly(vinyl alcohol)/poly(tetra fluoro ethylene) bilayer membrane system, Catalase was entrapped between poly(vinyl alcohol) membrane layer and poly(tetra fluoro ethylene) membrane layer outside of the galvanic type DO probe. Since poly(vinyl alcohol) membrane has non-porous, hydrophilic characteristics, the difference in hydrogen peroxide concentration between inside and outside of the membrane was therefore approximately 100 times. The developed hydrogen peroxide sensor has a wide linear range of hydrogen peroxide sensing more than 140 mM and favourable dynamic response characteristics. The sensor showed also good operational stability, rapid response time, and long life time.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abel, P., Muller, A., and Fischer, U. (1984), Biomed. Biophys. Acta, 5, 577–581.

    Google Scholar 

  • Aizawa, M., Karube, L, and Suzuki, S. (1974). Anal. Chim. Acta, 69, 431–437.

    Google Scholar 

  • Aptel, P., Challard, N., Cuny, J., and Neel, J. (1976). J. Membrane Sci., 1, 271–287.

    Google Scholar 

  • Brannon, M.L., and Nikolaos A.P. (1987). J. Membrane Sci., 32, 125–138.

    Google Scholar 

  • Chance, B. (1950). Biochem. J., 46, 387–402.

    Google Scholar 

  • Chance, B., and Maehly, A.C. (1955). In: Methods in Enzymology, S.P. Colowick and N.O. Kaplan, eds. vol.2. pp. 764–775, New York: Academic Press.

    Google Scholar 

  • Kirchner, J. R. (1987). In: Encyclopedia of Chemical Technology, Kirk-Othmer, eds. vol.13. pp. 12–19, New York: John Wiley and Sons.

    Google Scholar 

  • Murakami, T., Nakamoto, S., Kimura, T., Kuriyama, T., and Karube, I. (1986). Anal. Lett., 19, 1973–1986.

    Google Scholar 

  • Nakajima, R., and Yamazaki, I. (1987). J. Biol. Chem., 262, 2576–2581.

    Google Scholar 

  • Ogura, Y. (1955). Arch. Biochem. Biophys., 57, 288–300.

    Google Scholar 

  • Schonbaum, G. R., and Chance, B. (1976). In: The Enzymes, P. Boyer, eds. Vol.13. pp. 363–368, New York: Academic Press.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Seoul National University, 151-742, Seoul, Korea

    Hyun Joo & Young Je Yoo

Authors
  1. Hyun Joo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Young Je Yoo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Joo, H., Yoo, Y.J. Biosensor for the monitoring of hydrogen peroxide using poly(vinyl alcohol) membrane system. Biotechnol Tech 5, 453–458 (1991). https://doi.org/10.1007/BF00155492

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00155492

Keywords

Search

Navigation