Summary
Using cell recycle, the concentration of M. trichosporium OB3b was increased to more than 12g/L in continuous culture and biomass productivity was enhanced up to 0.708g/L/h. The optimal temperature and pH for soluble MMO activity were 35°C and 6.2–6.4. The Optimal concentrations of methanol and sodium formate, as cofactor regenerators, were 0.5mM and 10 mM, respectively, at which the soluble MMO activity was about 8 times enhanced.
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Lee, J., Soni, B.K. & Kelley, R.L. Enhancement of biomass production and soluble methane monooxygenase activity in continuous cultures of Methylosinus trichosporium OB3b. Biotechnol Lett 18, 897–902 (1996). https://doi.org/10.1007/BF00154617
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DOI: https://doi.org/10.1007/BF00154617