Abstract
A quasi-total loss of the bacterial hydroxylating activity was identified to be responsible for methanol production stop. Different strategies acting on the reaction mixture were implemented to apprehend the biocatalyst behavior in view to extend methanol production. Activity monitoring showed first that sodium formate addition did not maintain the biocatalyst activity and even disrupted bacterial equilibrium when added into the reaction mixture with still active biocatalysts. Reaction medium renewals had no influence on methanol production and highlighted a limited hydroxylating potential of the biocatalyst while addition of fresh biocatalysts in the reaction mixture resulted in methanol consumption. Finally, performing hydroxylation directly in the native bacterial culture appeared as a way to enhance methanol production by both release of intracellular methanol accumulated in the cells during cultivation and effective production by methane hydroxylation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aratani, Y., Y. Yamada, and S. Fukuzumi (2015) Selective hydroxylation of benzene derivatives and alkanes with hydrogen peroxide catalysed by a manganese complex incorporated into mesoporous silica-alumina. Chem. Commun. 51: 4662–4665.
Sivaramakrishna, A., P. Suman, E. V. Goud, S. Janardan, C. Sravani, C. S. Yadav, and H. S. Clayton (2012) Recent progress in oxidation of n-alkanes by heterogeneous catalysis. Res. Rev. Mat. Sci. Chem. 1: 75–103.
Park, D. H. and J. Lee (2013) Biological conversion of methane to methanol. Kor. J. Chem. Eng. 30: 977–987.
Rojo, F. (2009) Degradation of alkanes by bacteria. Environ. Microbiol. 11: 2477–2490.
Lieberman, R. L. and A. C. Rosenzweig (2004) Biological methane oxidation: Regulation, biochemistry, and active site structure of particulate methane monooxygenase. Crit. Rev. Biochem. Mol. Biol. 39: 147–164.
Anthony, C. (1986) Bacterial oxidation of methane and methanol. Adv. Microbial. Physiol. 27: 113–210.
Kim, H. G., G. H. Han, and S. W. Kim (2010) Optimization of lab scale methanol production by Methylosinus trichosporium OB3b. Biotechnol. Bioproc. Eng. 15: 476–480.
Lee, S. G., J. H. Goo, H. G. Kim, J. I. Oh, Y. M. Kim, and S. W. Kim (2004) Optimization of methanol biosynthesis from methane using Methylosinus trichosporium OB3b. Biotechnol. Lett. 26: 947–950.
Duan, C., M. Luo, and X. Xing (2011) High-rate conversion of methane to methanol by Methylosinus trichosporium OB3b. Bioresour. Technol. 102: 7349–7353.
Mehta, P. K., S. Mishra, and T. K. Ghose (1991) Methanol biosynthesis by covalently immobilized cells of Methylosinus trichosporium: Batch and continuous studies. Biotechnol. Bioeng. 37: 551–556.
Mehta, P. K., S. Mishra, and T. K. Ghose (1987) Methanol accumulation by resting cells of Methylosinus-trichosporium (I). J. Gen. Appl. Microbiol. 33: 221–229.
Furuto, T., M. Takeguchi, and I. Okura (1999) Semicontinuous methanol biosynthesis by Methylosinus trichosporium OB3b. J. Mol. Catal. a-Chem. 144: 257–261.
Sugimori, D., M. Takeguchi, and I. Okura (1995) Biocatalytic methanol production from methane with Methylosinus trichosporium OB3b: An approach to improve methanol accumulation. Biotechnol. Lett. 17: 783–784.
Pen, N., L. Soussan, M. P. Belleville, J. G. Sanchez Marcano, C. Charmette, and D. Paolucci-Jeanjean (2014) An innovative membrane bioreactor for methane biohydroxylation. Bioresour. Technol. 102: 7349–7353.
Ito, H., F. Mori, K. Tabata, I. Okura, and T. Kamachi (2014) Methane hydroxylation using light energy by the combination of thylakoid and methane monooxygenase. Rsc Adv. 4: 8645–8648.
Balasubramanian, R., S. M. Smith, S. Rawat, L. A. Yatsunyk, T. L. Stemmler, and A. C. Rosenzweig (2010) Oxidation of methane by a biological dicopper centre. Nature 465: 115–131.
Jiang, Y., P. C. Wilkins, and H. Dalton (1993) Activation of the hydroxylase of sMMO from Methylococcus-capsulatus (Bath) by hydrogen-peroxide. Biochim. Biophys. Acta 1163: 105–112.
Matsen, J. B., S. Yang, L. Y. Stein, D. Beck, and M. G. Kalyuzhnaya (2013) Global molecular analyses of methane metabolism in methanotrophic alphaproteobacterium, Methylosinus trichosporium OB3b. Part I: Transcriptomic study. Frontiers in Microbiol. 4.
Takeguchi, M., T. Furuto, D. Sugimori, and I. Okura (1997) Optimization of methanol biosynthesis by Methylosinus trichosporium OB3b: An approach to improve methanol accumulation. Appl. Biochem. Biotechnol. 68: 143–152.
Zollner, H. (1999) Handbook of Enzyme Inhibitors. 3rd ed. Wiley-VCH, NY, USA.
Adegbola, O. (2008) High cell density methanol cultivation of Methylosinus trichosporium OB3b, PhD thesis, Department of Chemical Engineering, Queens University, Ontario, Canada.
Chistoserdova, L., J. A. Vorholt, and M. E. Lidstrom (2005) A genomic view of methane oxidation by aerobic bacteria and anaerobic archaea. Genome Biol. 6:208.
Hanson, R. S. and T. E. Hanson (1996) Methanotrophic bacteria. Microbiol. Rev. 60: 439–471.
Li J, Gan JH, Mathews FS, Xia ZX (2011) The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase. Biochem. Biophys. Res. Commun. 406: 621–626.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Pen, N., Soussan, L., Belleville, MP. et al. Methane hydroxylation by Methylosinus trichosporium OB3b: Monitoring the biocatalyst activity for methanol production optimization in an innovative membrane bioreactor. Biotechnol Bioproc E 21, 283–293 (2016). https://doi.org/10.1007/s12257-015-0762-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12257-015-0762-0