Skip to main content
SpringerLink
Log in

A novel technique for entrapment of hybridoma cells in synthetic thermally reversible polymers

  • Published:
Biotechnology Techniques

Summary

A new technique for entrapment of cells in thermally reversible polymer (poly-N-vinylcaprolactam - PVCl) has been developed. Using stabilizers, such as ovalbumin, sodium carboxymethylcellulose or egg powder were necessary to give structures that were stable upon stirring carriers. Two hybridoma lines, in particular, 3B4 and 1A10 cells were entrapped in gel and produced monoclonal antibodies IgG and IgM classes, respectively, against Arabis mosaic virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Park, T.G., Hoffman, A.S., (1990). Biotechnol. Bioeng., 35(2), 152–159.

    Google Scholar 

  2. Iijima, S., Mano, T., Taniguchi, M., Kobayashi, T. (1988). Appl. Microbiol. Biotechnol. 28, 572–576.

    Google Scholar 

  3. Jilligan, K.J., Littlefield, S., Jarvis, A.P., (1988).In Vitro Cellular and Developmental Biology. 24, 35–41.

    Google Scholar 

  4. Kirsh, Yu.E, Suev, T.A., Karaputadze, T.M., Kobekov, V.V., Sinicina, L.A., Ostrovsky, S.A.,(1979). High molecular compounds A21, 12, 2734 (in Russ.).

    Google Scholar 

  5. Plechko, T.N., Kirillov, A.V., Ambrosova, S.M., Borisova, O.V. (1990). Bioorganic. Chemistry (in Russ.) in press

  6. Markvicheva, E.A., Donova, M.V., Kuzkina, I.F., Pashkin, I.I.,(1990). Abstr. 7th International Conf.Young Scient. on Organic and Biol. Chem., Varna, Bulgaria (17–23 September, 1990), 49–51.

Download references

Author information

Authors and Affiliations

  1. Shemyakin Institute of Bioorganic Chemistry USSR Academy of Sciences, ul. Miklukho-Maklaya, 16/10, 117871, Moscow, USSR

    Markvicheva E. A., Kuz'kina I. F., Pashkin I. I., Plechko T. N., Kirsh Yu. E. & Zubov V. P.

  2. Lomonosov Institute of Fine Chemical Technology, M.Pirogovskaya ul., 1, Moscow, USSR

    Kuz'kina I. F., Pashkin I. I. & Kirsh Yu. E.

Authors
  1. Markvicheva E. A.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kuz'kina I. F.
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pashkin I. I.
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Plechko T. N.
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kirsh Yu. E.
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zubov V. P.
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Markvicheva, E.A., Kuz'kina, I.F., Pashkin, I.I. et al. A novel technique for entrapment of hybridoma cells in synthetic thermally reversible polymers. Biotechnol Tech 5, 223–226 (1991). https://doi.org/10.1007/BF00152786

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00152786

Keywords

Search

Navigation