Skip to main content
SpringerLink
Log in

Loading and release of a model high-molecular-weight protein from temperature-sensitive micro-structured hydrogels

  • Research Letter
  • Published:
MRS Communications Aims and scope Submit manuscript

Abstract

The authors prepared a micro-structured, thermosensitive hydrogel with N-isopropylacrylamide microgels with a lower critical solution temperature (LCST) of 32 °C dispersed on a matrix of N-isopropylacrylamide-co-dimethylacrylamide with an LCST at 40 °C. Incubation of the hydrogel at 33 °C in a solution of fluorescein-albumin induced loading of the protein. The protein was not loaded at a temperature below the LCST of the microgels (4 °C), suggesting that the shrinkage of the microgels followed by the formation of micropores within the hydrogel matrix is a prerequisite for protein loading. A sustained and complete release of the loaded protein was obtained at 37 °C.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.
Figure 4.

Similar content being viewed by others

References

  1. R. Zaman, R.A. Islam, N. Ibnat, I. Othman, A. Zaini, C.Y. Lee, and E.H. Chowdhury: Current strategies in extending half-lives of therapeutic proteins. J. Control. Release 301, 179 (2019).

    Article  Google Scholar 

  2. P.K. Deb, O. Al-Attraqchi, B. Chandrasekaran, and A. Paradkar: Basic Fundamentals of Drug Delivery. Advances in Pharmaceutical Product Development and Research. Chapter 16 - Protein/Peptide Drug Delivery Systems: Practical Considerations in Pharmaceutical Product Development (Academic Press, Boston, 2019), pp. 651. https://doi.org/10.1016/B978-0-12-817909-3.00016-9.

    Google Scholar 

  3. T. Vermonden, R. Censi, and W.E. Hennink: Hydrogels for protein delivery. Chem. Rev. 112, 2853 (2012).

    Article  CAS  Google Scholar 

  4. M. Yu, J. Wu, J. Shi, and O.C. Farokhzad: Nanotechnology for protein delivery: overview and perspectives. J. Control. Release 240, 24 (2016).

    Article  CAS  Google Scholar 

  5. Y. Lu, W. Sun, and Z. Gu: Stimuli-responsive nanomaterials for therapeutic protein delivery. J. Control. Release 194, 1 (2014).

    Article  CAS  Google Scholar 

  6. L.A. Sharpe, A.M. Daily, S.D. Horava, and N.A. Peppas: Therapeutic applications of hydrogels in oral drug delivery. Expert Opin. Drug Deliv. 11, 901 (2014).

    Article  CAS  Google Scholar 

  7. J. Li and D.J. Mooney: Designing hydrogels for controlled drug delivery. Nat. Rev. Mater. 1, 16071 (2016).

    Article  CAS  Google Scholar 

  8. A. Vashist, A. Vashist, Y.K. Gupta, and S. Ahmad: Recent advances in hydrogel based drug delivery systems for the human body. J. Mater. Chem. B 2, 147 (2014).

    Article  CAS  Google Scholar 

  9. L.D. Taylor and L.D. Cerankowski: Preparation of films exhibiting a balanced temperature dependence to permeation by aqueous solutions–a study of lower consolute behavior. J. Polym. Sci. Polym. Chem. Ed. 13, 2551 (1975).

    Article  CAS  Google Scholar 

  10. J.Y. Wu, S.Q. Liu, P.W.S. Heng, and Y.Y. Yang: Evaluating proteins release from, and their interactions with, thermosensitive poly (N-isopropylacrylamide) hydrogels. J. Control. Release 102, 361 (2005).

    Article  CAS  Google Scholar 

  11. T. Tanaka and D.J. Fillmore: Kinetics of swelling of gels. J. Chem. Phys. 70, 1214 (1979).

    Article  CAS  Google Scholar 

  12. J. Museh, S. Schneider, P. Lindner, and W. Richtering: Unperturbed volume transition of thermosensitive poly-(N-isopropylacrylamide) microgel particles embedded in a hydrogel matrix. J. Phys. Chem. B 112, 6309 (2008).

    Article  Google Scholar 

  13. K. Palomino, K.A. Suarez-Meraz, A. Serrano-Medina, A. Olivas, E.C. Samano, and J.M. Cornejo-Bravo: Microstructured poly(N-isopropylacrylamide) hydrogels with fast temperature response for pulsatile drug delivery. J. Polym. Res 22(199), 1–9 (2015). https://doi.org/10.1007/s10965-015-0841-0.

    CAS  Google Scholar 

  14. K. Palomino, J.M. Cornejo-Bravo, H. Magaña, and A. Serrano-Medina: Microstructured hydrogels with modulated transition temperature for positive control release. Dig. J. Nanomater. Biostruct. 13, 141 (2018).

    Google Scholar 

  15. C. Obeso-Vera, J.M. Cornejo-Bravo, A. Serrano-Medina, and A. Licea-Claverie: Effect of crosslinkers on size and temperature sensitivity of poly(N-isopropylacrylamide) microgels. Polym. Bull 70, 653 (2013).

    Article  CAS  Google Scholar 

  16. S. Dash, P.N. Murthy, L. Nath, and P. Chowdhury: Kinetic modeling on drug release from controlled drug delivery systems. Acta Pol. Pharm. 67, 217 (2010).

    CAS  Google Scholar 

  17. S.T. Koshy, D.K.Y. Zhang, J.M. Grolman, A.G. Stafford, and D.J. Mooney: Injectable nanocomposite cryogels for versatile protein drug delivery. Acta Biomater. 65, 36 (2018).

    Article  CAS  Google Scholar 

  18. V.H. Perez-Luna and O. Gonzalez-Reynoso: Encapsulation of biological agents in hydrogels for therapeutic applications. Gels 4, 61 (2018).

    Article  Google Scholar 

  19. M.W. Sabaa, D.H. Hanna, M.H. Abu Elella, and R.R. Mohamed: Encapsulation of bovine serum albumin within novel xanthan gum based hydrogel for protein delivery. Mater. Sci. Eng. C 94, 1044 (2019).

    Article  CAS  Google Scholar 

  20. R. Egbu, S. Brocchini, P.T. Khaw, and S. Awwad: Antibody loaded collapsible hyaluronic acid hydrogels for intraocular delivery. Eur. J. Pharm. Biopharm. 124, 95 (2018).

    Article  CAS  Google Scholar 

  21. S. Dutta, P. Samanta, and D. Dhara: Temperature, pH and redox responsive cellulose based hydrogels for protein delivery. Int. J. Biol. Macromol. 87, 92 (2016).

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors acknowledge funding from 20th Internal Call for the Support of Research Projects from UABC, Support for the Strengthening of Academic Bodies from PRODEP, SEP's Grant Program to support New Full Time Professors PRODEP 2018 (UABC-PTC-735), and from the Third Special Internal Call for Support to Research Projects (UABC-3902). M.A.-M. acknowledges funding from CONACyT (Mexico) through Research Projects INFR-2015-251863 and PDCPN-2015-89.

Author information

Authors and Affiliations

  1. Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Calzada Universidad 14418, Parque industrial Internacional, C.P. 22427, Tijuana, B.C., Mexico

    Kenia Palomino, Héctor Magaña, Samuel G. Meléndez-López & José M. Cornejo-Bravo

  2. Facultad de Medicina y Psicología, Universidad Autónoma de Baja California, Calzada Universidad 14418, Parque industrial Internacional, C.P. 22427, Tijuana, B.C., Mexico

    Aracely Serrano-Medina

  3. Cátedras CONACyT-Tecnológico Nacional de México/I. T. Tijuana, Centro de Graduados e Investigación en Química-Grupo de Biomateriales y Nanomedicina, Blvd. Alberto Limón Padilla S/N, 22510, Tijuana, B.C., Mexico

    Manuel Alatorre-Meda

  4. Facultad de Odontología Campus Tijuana, Universidad Autónoma de Baja California, Calzada Universidad 14418, Parque industrial Internacional, 22390, Tijuana, B.C., Mexico

    Eustolia Rodríguez-Velázquez

  5. Tecnológico Nacional de México/I. T. Tijuana, Centro de Graduados e Investigación en Química - Grupo de Biomateriales y Nanomedicina, Blvd. Alberto Limón Padilla S/N, 22510, Tijuana, B.C., Mexico

    Eustolia Rodríguez-Velázquez

  6. Tecnológico Nacional de México/I. T. Tijuana, Centro de Graduados e Investigación en Química, Blvd. Alberto Limón Padilla S/N, 22510, Tijuana, B.C., Mexico

    Ignacio Rivero

Authors
  1. Kenia Palomino
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Héctor Magaña
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Samuel G. Meléndez-López
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. José M. Cornejo-Bravo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Aracely Serrano-Medina
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Manuel Alatorre-Meda
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Eustolia Rodríguez-Velázquez
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ignacio Rivero
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to José M. Cornejo-Bravo.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Palomino, K., Magaña, H., Meléndez-López, S.G. et al. Loading and release of a model high-molecular-weight protein from temperature-sensitive micro-structured hydrogels. MRS Communications 9, 1041–1045 (2019). https://doi.org/10.1557/mrc.2019.87

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1557/mrc.2019.87

Search

Navigation