Skip to main content
SpringerLink
Log in

Determining molecular weight distributions of lignins and their biodegradation products by gel filtration on a high-performance acarose column with a mixed ethanol-aqueous alkali solvent

  • Published:
Biotechnology Techniques

Summary

Molecular weight distributions of 14C-labelled alkali-soluble lignins and their biodegradation products can be quickly and conveniently determined by gel filtration on a Pharmacia Superose 12 column. Elution with a 1:1 mixture of ethanol and 0.1 M NaOH prevents adsorption of the lignins on the gel.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Connors, W.J. (1978). Holzforschung 32, 145–147.

    Google Scholar 

  • Connors, W.J., Lorenz, L.F., Kirk, T.K. (1978). Holzforschung 32, 106–108.

    Google Scholar 

  • Connors, W.J., Sarkanen, S., McCarthy, J.L. (1980). Holzforschung 34, 80–85.

    Google Scholar 

  • Haemmerli, S.D., Leisola, M.S.A., Fiechter, A. (1986). FEMS Microbiol. Lett. 35, 33–36.

    Google Scholar 

  • Hüttermann, A. (1978). Holzforschung 32, 108–111.

    Google Scholar 

  • Hüttermann, A., Herche, C., Haars, A. (1980). Holzforschung 34, 64–66.

    Google Scholar 

  • Kern, H.W., Kirk, T.K. (1987). Appl. Environm. Microbiol. 53, 2242–2246.

    Google Scholar 

  • Kirk, T.K., Brunow, G. (1988). Methods in Enzymology 161, 65–73.

    Google Scholar 

  • Lundquist, K., Kirk, T.K. (1980). Tappi Journal 63, 80–82.

    Google Scholar 

  • Pellinen, J., Salkinoja-Salonen, M. (1985a). J. Chromatography 322, 129–138.

    Google Scholar 

  • Pellinen, J., Salkinoja-Salonen, M. (1985b). J. Chromatography 328, 299–308.

    Google Scholar 

  • Reid, I.D., Deschamps, A.M. (1991). Can. J. Bot. 69, 147–155.

    Google Scholar 

  • Zimmermann, W., Paterson, A., Broda, P. (1988). Methods in Enzymology 6, 191–199.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Research Council of Canada, Biotechnology Research Institute, 6100 Ave. Royalmount, H4P 2R2, Montreal, Quebec, Canada

    Ian D. Reid

Authors
  1. Ian D. Reid
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Reid, I.D. Determining molecular weight distributions of lignins and their biodegradation products by gel filtration on a high-performance acarose column with a mixed ethanol-aqueous alkali solvent. Biotechnol Tech 5, 215–218 (1991). https://doi.org/10.1007/BF00152784

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00152784

Keywords

Search

Navigation