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Immobilization of β-galactosidases from Thermus aquaticus YT-1 for oligosaccharides synthesis

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Biotechnology Techniques

Summary

β-galactosidases of Thermus aquaticus YT-1, exhibiting a galactosyl transferase activity, were immobilized using different techniques. Entrapment in agarose or gellan gum beads was unsuitable for enzyme immobilization due to enzyme leakage. A technique that efficiently immobilized the enzymes was developed using glutaraldehyde co-crosslinking of β-galactosidases with bovine serum albumin, followed by entrapment in agarose beads.

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Authors and Affiliations

  1. Centre de recherche STELA, Pavillon Paul-Comtois, Université Laval, Ste-Foy, Qc., G1K 7P4, Canada

    J. L. Berger & C. Lacroix

  2. Dept. of Food Science, McGill University, Ste-Anne de Bellevue, Qc., H9X 3V9, Lakeshore, Canada

    J. L. Berger & B. H. Lee

  3. F.R.D.C., Agriculture Canada, St-Hyacinthe, Qc., J2S 8E3, Canada

    B. H. Lee

Authors
  1. J. L. Berger
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  2. B. H. Lee
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  3. C. Lacroix
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Berger, J.L., Lee, B.H. & Lacroix, C. Immobilization of β-galactosidases from Thermus aquaticus YT-1 for oligosaccharides synthesis. Biotechnol Tech 9, 601–606 (1995). https://doi.org/10.1007/BF00152452

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  • DOI: https://doi.org/10.1007/BF00152452

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