Summary
The water-soluble solvent dimethylsulfoxide proved to be inhibitory to the enzymatic glucosylation of crocetin catalyzed by cell-free extracts of saffron (Crocus sativus L.) callus cultures. This problem was circumvented by encapsulating crocetin into maltosyl-β-cyclodextrin at a 1:3 and a 1:6 ratio which made it possible to carry out the reaction with up to 1,750 and 2,500 μM crocetin respectively.
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Cormier, F., Dufresne, C. & Dorion, S. Enhanced crocetin glucosylation by means of maltosyl-β-cyclodextrin encapsulation. Biotechnol Tech 9, 553–556 (1995). https://doi.org/10.1007/BF00152442
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DOI: https://doi.org/10.1007/BF00152442