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Separation of cephalosporin C and desacetyl cephalosporin C by high speed counter-current chromatography in aqueous two-phase systems

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Biotechnology Techniques

Summary

In the recovery of cephalosporin C (CPC) from fermentation broth, the separation of desacetyl cephalosporin C (DAC) is a major concern. Multistage extraction in aqueous two-phase systems, mainly PEG/ammonium sulfate systems, proved to be promising. In preparative scale operation, high speed counter-current chromatography (HSCCC) with eccentric columns was used with aqueous two-phase systems to obtain baseline resolution of CPC and DAC. Solvents (e.g. 5% acetone) or neutral salts (e.g. 1.45% KSCN) added into aqueous two-phase systems enhanced the separation efficiency. Operation parameters of HSCCC such as rotational speed and mobile phase flow rate can affect the retention of the stationary phase and HETP.

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References

  • Sutherland, I.A., Heywood-Waddington, D., Ito, Y.(1987), J. Chromatogr., 384,197–207.

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  • Yang, W.Y., Lin,C.D.,Chu,I.M.,Lee,C.J., (1994), Biotechnol. Bioeng. 43, 439–445.

    Google Scholar 

  • Wildfeuer, M.E., (1985), Approaches to cephalosporin C purification from fermentation broth, in Purification of Fermentation Products, Leroith, D., Schiloach, J., Leaby, T.J. eds, pp155–174, ACS Symp. Ser. 271, Washington D.C.: American Chemical Society.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Tsing Hua University, 300, Hsinchu, R.O.C.

    Po-Chun Lin & I-Ming Chu

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  1. Po-Chun Lin
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  2. I-Ming Chu
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Lin, PC., Chu, IM. Separation of cephalosporin C and desacetyl cephalosporin C by high speed counter-current chromatography in aqueous two-phase systems. Biotechnol Tech 9, 549–552 (1995). https://doi.org/10.1007/BF00152441

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  • DOI: https://doi.org/10.1007/BF00152441

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