Summary
Batch cultivation of Aspergillus wentii, Wehmer, Pt 2804 was carried out on Mandels and Reese (1957) medium containing 3% cellulose and 0.3% peptone at 30°C in 14 and 30 liter Bioreactors. Use of a 48 hours old inoculum (10% v/v), 0.5 vvm aeration at 400–800 rpm and maintenance of dissolved oxygen and pH above 50% saturation level and between 5.2 to 4.0 respectively, produced 10.58 g cells/lit and 61.9 IU/1/hr cellobiase productivity.
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References
Allen, A., and Sternberg, D., (1980), Biotech. Bioeng. Symp. No.10, p.189
Bergmeyer, H. U., and Bent, E. (1974) In: Methods of Enzymatic Analysis, H. U. Bergmeyer, ed., Vol.3, p.205
Bisaria, V. S. (1978), Ph.D. Thesis, Chemical Engineering Department, IIT Delhi
Bissett, F., and Sternberg, D. (1978), Appl. Environ. Microbiol., 35, p.750
Enari, T-M., Niku-paavola, M-L., and Nummi, M., (1980), In: Second International Symposium on Bioconversion and Biochemical Engineering, T. K. Ghose, ed., (in press)
Herbert, D., Phillips, P. J., and Strage, R. E. (1971), J. Bacteriology, 5B, p.244
Mandels, M., Andreotti, R. E., and Charles Roche (1977), In: Proceedings of Bioconversion Symposium, T. K. Ghose, ed., Thomson Press, India
Mandels, M., and Reese, E. T. (1957), J. Bacteriology, 73 p.648
Scott, T. A. Jr., and Melvin, E. H. (1953), Anal.Chem., 25, p.1653
Srivastava, S. K., Ramachandran, K. B. and Gopalkrishnan, K. S. (1981) - under communication
Sternberg, D. (1976), Appl. Environ. Microbiol., 31 p.648
Yamanaka, Y., and Wilke, C. R. (1975), Lawrence Berkeley Laboratory, University of California, LBL 441, p. 14
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Srivastava, S.K., Ramachandran, K.B. & Gopalkrishnan, K.S. β - glucosidase production by Aspergillus wentii in stirred tank bioreactors. Biotechnol Lett 3, 477–480 (1981). https://doi.org/10.1007/BF00147557
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DOI: https://doi.org/10.1007/BF00147557