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Prevalence of Cryptosporidium in children with enteritis in Southern Italy

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Abstract

Cryptosporidium parvum is a protozoan which causes self-limiting diarrhea in immunocompetent subjects, and severe life-threatening disease in immunocompromised patients. Cryptosporidiosis is more common in developing countries and in infants. In this paper we have evaluated the prevalence of C. parvum in 368 hospitalized children with enteritis, of whom 359 were immunocompetent and 9 HIV-infected. Stool specimens were concentrated by sedimentation and stained with a modified Ziehl-Neelsen method. Cryptosporidium parvum oocysts were found in 7 (1.90%)out of 368 subjects. Six of these were immunocompetent (with an infection rate in this population of 1.67%) and 1 HIV-infected, asymptomatic except for diarrhea. In all children symptoms of enteritis and oocyst excretion cleared within 10 days. These results indicate that the prevalence of C. parvum as a causative agent of diarrheal illness in hospitalized immunocompetent children is rather low in our region (Apulia, South Italy).

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References

  1. Crawford F. Human cryptosporidiosis. Crit Rev Microbiol 1988; 16: 113–159.

    Google Scholar 

  2. Ditrich O, Palkovic L, Sterba J, Prokopic J, Loudova J, Giboda M. The first finding of Cryptosporidium baileyi in man. Parasitol Res 1991; 77: 44–47.

    Google Scholar 

  3. Tizzer E. A sporozoan found in the peptic glands of the common mouse. Proc Soc Exp Biol Med 1907; 5: 12–13.

    Google Scholar 

  4. Nime F, Burek J, Page D, Hoescher M, Yardley J. Acute enterocolitis in a human being infected with the protozoan Cryptosporidium. Gastroenterology 1976; 70: 592–598.

    Google Scholar 

  5. Current W, Garcia LS. Cryptosporidiosis. Clin Microbiol Rev 1991; 4: 325–358.

    Google Scholar 

  6. Addy P, Aikins-Bekoe P. Cryptosporidiosis in diarrhoeal children in Ghana. Lancet 1986; i: 735.

    Google Scholar 

  7. Baxby D, Hart C. The incidence of cryptosporidiosis: A two-year prospective survey in a children's hospital. J Hyg (Camb) 1986; 96: 107–111.

    Google Scholar 

  8. Berkowitz FE, Vallabh W, Buqwana A, Heney C. Cryptosporidiosis in black South African children. S Afr Med J 1988; 74: 272–273.

    Google Scholar 

  9. Hojlying N, Molbak K, Jepsen S, Hansson A. Cryptosporidiosis in Liberian children. Lancet 1984; ii: 734.

    Google Scholar 

  10. Marshall A, AL-Jumaili I, Fenwick G, Bint A, Record C. Cryptosporidiosis in patients at a large teaching hospital. J Clin Microbiol 1987; 25: 172–173.

    Google Scholar 

  11. Mølbak K, Hojlyng N, Gottschau A, Correia Sá JC, Ingholt L, da Silva APJ, Aaby P. Cryptosporidiosis in infancy and childhood mortality in Guinea Bissau, West Africa. Br Med J 1993; 307: 417–420.

    Google Scholar 

  12. Perez-Schael I, Boher Y, Mata L, Perez M, Tapia F. Cryptosporidiosis in Venezuelan children with acute diarrhoea. Am J Trop Med Hyg 1985; 34: 721–722.

    Google Scholar 

  13. Wittenberg DF, Smith EG, van den Ende J, Recker PJ. Cryptosporidium-associated diarrhea in children. Ann Trop Paediatr 1987; 7: 113–117.

    Google Scholar 

  14. Bogaerts J, Lepage P, Rouvroy D, Vandepitte J. Cryptosporidium spp., a frequent cause of diarrhoea in Central Africa. J Clin Microbiol 1984; 20: 874–876.

    Google Scholar 

  15. Caprioli A, Gentile G, Baldassarri L, Bisicchia R, Romoli E, Donelli G. Cryptosporidium as a common cause of childhood diarrhoea in Italy. Epidem Infect 1989; 102: 537–540.

    Google Scholar 

  16. Freidank H, Kist M. Cryptosporidia in immunocompetent patients with gastroenteritis. Eur J Clin Microbiol 1987; 6: 56–59.

    Google Scholar 

  17. Heidl M, Hering L, Scholz H. Cryptosporidiosis in children. Infection 1986; 14: 173–176.

    Google Scholar 

  18. Lopez-Brea M, Garcia-Picazo L, Del Rey M. Cryptosporidium in children and adults in Madrid. J Infection 1986; 158: 647–648.

    Google Scholar 

  19. Miron D, Kenes Y. Cryptosporidiosis in children. Harefuah 1990; 15: 315–318.

    Google Scholar 

  20. Ritchie LS. An ether sedimentation technique for routine stool examinations. Bull US Army Med Dept 1948; 8: 326.

    Google Scholar 

  21. Casemore DP, Armstrong M, Jackson B. Screening for Cryptosporidium in stools. Lancet 1984; i: 734–735.

    Google Scholar 

  22. Anonymous. Immunodeficiency and cryptosporidiosis. Demonstration at the Royal College of Physicians of London. Br Med J 1980; 281: 1123–1127.

  23. Ma P, Soave R. Three-step stool examination for cryptosporidiosis in 10 homosexual men with protracted watery diarrhea. J Infect Dis 1983; 147: 824–828.

    Google Scholar 

  24. Sterling C, Arrowood M. Detection of Cryptosporidium sp. infections using a direct immunofluorescent assay. Pediatr Infect Dis 1986; 5: S139–142.

    Google Scholar 

  25. Arrowood M, Sterling C. Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection. J Clin Microbiol 1989; 27: 1490–1495.

    Google Scholar 

  26. Hojlyng N, Molbak K, Jepsen S. Cryptosporidium spp., a frequent cause of diarrhea in Liberian children. J Clin Microbiol 1986; 23: 1109–1113.

    Google Scholar 

  27. Cruz J, Cano F, Càceres P, Chew F, Pareja G. Infection and diarrhea caused by Cryptosporidium sp. among Guatemalan infants. J Clin Microbiol 1988; 26: 88–91.

    Google Scholar 

  28. Hart CA, Baxby D, Blundell N. Gastroenteritis due to Cryptosporidium: A prospective survey in a children's hospital. J Infect 1984; 9: 264–270.

    Google Scholar 

  29. Phillips AD, Thomas AG, Walker-Smith JA. Cryptosporidium, chronic diarrhoea and the proximal small intestinal mucosa. Gut 1992; 33: 1057–1061.

    Google Scholar 

  30. Arnaud-Battandier F, Naceri M, Maurage C. Cryptosporidiosis in immunocompetent patients. Engl J Med 1985; 313: 1019.

    Google Scholar 

  31. Desgrandchamps D, Minzinger J. Infectious gastroenteritis in the immunocompetent child. Significance of Cryptosporidium spp. and Aeromonas spp. Schweiz. Med Wochenschr 1989; 119: 276–281.

    Google Scholar 

  32. Mai Nguyen X. Cryptosporidial diarrhoea in children. Infection 1987; 15: 444–446.

    Google Scholar 

  33. Thomson MA, Benson JWT, Wright PA. Two year study of Cryptosporidium infection. Arch Dis Child 1987; 62: 559–563.

    Google Scholar 

  34. Moretti M, Fiorio M, Antonelli S, Baldelli F, Stagni G, Pauluzzi S. Indagine sulla presenza del Cryptosporidium in Umbria. Boll Ist Sieroter Milan 1988; 67: 226–231.

    Google Scholar 

  35. Brandonisio O, Angarano G, Altamura M, Locaputo S, Pastore G, Canestri-Trotti G. Cryptosporidiosis in the acquired immunodeficiency syndrome: A case report. Eur J Epidemiol 1987; 3: 442–444.

    Google Scholar 

  36. Brandonisio O, Maggi P, Panaro MA, Bramante L, Di coste A, Angarano G. Prevalence of cryptosporidiosis in HIV-infected patients with diarrhoeal illness. Eur J Epidemiol 1993; 9: 190–194.

    Google Scholar 

  37. Garcia LS, Brewer TC, Bruckner DA. Incidence of Cryptosporidium in all patients submitting stool specimens for ova and parasite examination: Mono clonal antibody-IFA method. Diagn Microbiol Infect Dis 1989; 11: 25–27.

    Google Scholar 

  38. Casemore DP. Laboratory methods for diagnosing cryptosporidiosis. J Clin Path 1991; 44: 445–451.

    Google Scholar 

  39. Gomez Morales MA, Ausiello CM, Urbani F, Pozio E. Crude extract and recombinant protein of Cryptosporidium parvum oocysts induce proliferation of human peripheral blood mononuclear cells in vitro. J Infect Dis 1995; 172.

  40. Kuhls TL, Mosier DA, Crawford DL, Griffis J. Seroprevalence of cryptosporidial antibodies during infancy, childhood, and adolescence. Clin Infect Dis 1994; 18: 731–735.

    Google Scholar 

  41. Gomez Morales MA, Pozio E, Croppo GP. Serodiagnosis of cryptosporidiosis in Italian HIV-positive patients by means of an oocyst soluble antigen in an ELISA. J Infection 1992; 25: 229–236.

    Google Scholar 

  42. Mathan MM, Venkatesan S, George R, Mathew M, Mathan VJ. Cryptosporidium and diarrhea in Southern Indian children. Lancet 1985; ii: 1172–1175.

    Google Scholar 

  43. Shahid NS, Rahaman ASMH, Anderson BC, Mata LJ, Sanyal SC. Cryptosporidiosis in Bangladesh. Br Med J 1985; 290: 114–115.

    Google Scholar 

  44. Flanigan T, Whalen C, Turner J, Soave R, Toerner J, Havlir D, Kotler D. Cryptosporidium infection and CD4 counts. Ann Intern Med 1992; 116: 840–842.

    Google Scholar 

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Authors and Affiliations

  1. Istituto di Microbiologia Medica, Università di Bari, Policlinico, Piazza G. Cesare, 1-70124, Bari, Italy

    O. Brandonisio, A. Marangi & M. I. Natalicchio

  2. Istituto di Anatomia Umana Normale, Università di Bari, Bari, Italy

    M. A. Panaro & R. Marzio

  3. Ospedule Pediatrico Giovanni XXIII, I Divisione Malattie Infettive, Bari, Italy

    P. Zizzadoro & U. De Santis

Authors
  1. O. Brandonisio
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  2. A. Marangi
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  3. M. A. Panaro
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  4. R. Marzio
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  5. M. I. Natalicchio
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  6. P. Zizzadoro
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  7. U. De Santis
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Brandonisio, O., Marangi, A., Panaro, M.A. et al. Prevalence of Cryptosporidium in children with enteritis in Southern Italy. Eur J Epidemiol 12, 187–190 (1996). https://doi.org/10.1007/BF00145505

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