Summary
Using a quadrupole mass spectrometer (QMS), we continuously monitored N2, O2, CO2, and ethanol in the outlet gas of a batch fermentor. A computer was used to control the QMS, perform correction calculations and account for interferences between some ion peaks. The ability to continously scan multiple peaks and to use N2 as an internal standard provided rapid and accurate monitoring of fermentation performance. Over the range of interest, a linear response was obtained for all the compounds measured by the QMS.
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References
Churchill, S.W. (1974). The interpretation and use of rate data. In: The Rate Concept, p. 510, Scripta Publishing Co: Washington D.C.
Delpy, D.T. and Parker, D. (1975). Lancet, 3 May, 1016.
Eight Peak Index of Mass Spectra (1974). p. 1493, Mass Spectrometry Data Centre: Awre, U.K.
Maxon, W.D. and Johnson, M.J. (1953). Ind. Chem. Eng. 45, 2554–2560.
Miller, G.L. (1959). Anal. Chem. 31, 426–428.
Mieure, J.P., Mappes, G.W., Tucker, E.S. and Dietrich, M.W. (1976). Separation of trace organic compounds from water. In: Identification & Analysis of Organic Pollutants in Water, L.H. Keith, ed., pp. 113–133, Ann Arbor Science: Ann Arbor, MI.
Muysers, K. and Smidt, V. (1972). Clinical uses of mass spectrometry. In: Biomedical Applications of Mass Spectrometry, G.R. Waller, ed., pp. 601–622. Wiley-Interscience: New York.
Reuss, M., Piehl, H. and Wagner, F. (1975). Europe. J. Appl. Microbiol., 1, 323–325.
Sodal, I. E. and Swanson, G.D. (1980). Mass spectrometer in patient monitoring. In: Essential Non-invasive Monitoring, J.S. Gravenstein, R.S. Newbower, A.K. Ream and N.T. Smith, eds., pp. 217–234, Grun and Stratton: New York.
Wang, H.Y., Cooney, C.L. and Wang, D.I.C. (1977). Biotechnol. Bioeng. 19, 69–86.
Weaver, J.C., Mason, M.K., Jarrell, J.A. and Peterson, J.W. (1976). Biochem. Biophis. Acta, 438, 296–303.
Weaver, J.C. and Abrams, J.H. (1979). Rev. Sci. Inst. 50, 478–481.
Weaver, J.C., Perley, C.R., Reames, F.M. and Cooney, C.L. (1980). Biotechnol. Lett., 2, 133–137.
Westover, L.B., Tou, J.C. and Mark, J.H. (1974). Anal. Chem., 46, 568–571.
Woldering, S., Owens, F. and Woolford, D.C. (1976). Science, 153, 885–887.
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Pungor, E., Perley, C.R., Cooney, C.L. et al. Continuous monitoring of fermentation outlet gas using a computer coupled MS. Biotechnol Lett 2, 409–414 (1980). https://doi.org/10.1007/BF00144246
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DOI: https://doi.org/10.1007/BF00144246