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The effect of pH on hydrogen production with Citrobacter intermedius

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Summary

Citrobacter intermedius was grown in a 14-liter fermenter under batch anaerobic conditions at the following controlled pH values: 5, 5.75, 6.0, 6.5, 7.0, 7.5, and 8.0. The growth medium was a glucose mineral salts medium with 0.1% ammonium sulfate as the source of sulfur. The optimum pH for H2 production was 5.75 and 6.0 which gave a yield of 1.1 moles H2/mole glucose. The optimum H2-productivity was 144 moles H2 per hour at pH 6.0.

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Author notes

  1. J. D. Brosseau

    Present address: Department of Microbiology, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada

Authors and Affiliations

  1. Biochemical and Food Engineering, University of Western Ontario, London, Ontario, Canada

    J. D. Brosseau, N. Kosaric & J. E. Zajic

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  1. J. D. Brosseau
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  2. N. Kosaric
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  3. J. E. Zajic
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Brosseau, J.D., Kosaric, N. & Zajic, J.E. The effect of pH on hydrogen production with Citrobacter intermedius . Biotechnol Lett 2, 93–98 (1980). https://doi.org/10.1007/BF00142032

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