Abstract
Isolation of Histoplasma farciminosum from five horses, showing typical signs of histoplasmosis farciminosi (epizootic lymphangitis) was successfully attempted. The mycelial form of H. farciminosum was isolated on Sabouraud dextrose agar enriched with 2.5% glycerol, brain heart infusion (BHI) agar enriched with 10% horse blood and PPLO dextrose glycerol agar. The last medium proved to be the most effective, both for primary isolation and subculturing of the fungus. It was found that on primary isolation, the lag phase of the mycelial form of the fungus was relatively long, involving 4–8 weeks at 25°C. Colonies of the mycelial form of H. farciminosum appeared on subculture as a yellowish, light brown to deep brown, convoluted, waxy, cauliflower-like growth tending to form scant aerial growth.
Conversion of the mycelial form to the yeast form of H. farciminosum was successful by subculturing either on BHI agar with 5% blood or on Pine's medium and incubating at 35–37°C. Complete conversion to the yeast form was achieved only after 4–5 repeated serial transfers onto fresh media every 8 days. The yeast colonies were flat, raised, slightly or deeply wrinkled, white to light gray to grayish brown, and were pasty in consistency.
Similar content being viewed by others
References
RivoltaS. and MicelloneI. (1883): Del farcino criptococchio. - Giorn. di Anat. Fisiol. e Patol. d. Animali Domestici, 15: 143–162.
TokishigeH. (1896): Uber pathogene Blastomyceten.- Zbl. f. Bakt. E Abt. Orig., Bd., 19: 105–116.
Rocha LimaH.da (1912): Beitrag zur Kenntnis der Blastomykosen. Lymphangitis epizootica und Histoplasmosis. - Centralblt. Bakt. I, 67: 233–249.
BullenJ.J. (1949): The yeast-like form of Cryptococcus farciminosus (Rivolta): (Histoplasma farciminosum).- J. Pathol. Bacteriol., 61: 117–120.
SmithH.A. and JonesT.C. (1961): Veterinary Pathology. 2nd Ed. pp. 1068, Lea and Febiger, Philadelphia.
SinghH. (1966): Studies on epizootic lymphagitis. Study of clinical cases and experimental transmission. -Indian J. Vet. Sci., 36: 45–59.
SanfeliceF. (1906): Uber die Pathogene Wirkung der Blastomyceten. - Zeitschrift f. Hygiene u. Infektionkrankheiten, 54: 229–324.
OrlovF.M. (1979): Infeksionni u Invasioni bolenzni loshadei. - Moscow, Kolos, 209–219.
BloodD.C., HendersonT.A. and RodostetsO.M. (1979): Veterinary Medicine, 5th Ed. The English Language Book Society and Bailliere Tindall., London, 724–726.
Abou-GabalM., HassanF.K., Al-SaidA.A. and Al-KarimK.A. (1983): Study on equine histoplasmosis lymphangitis. - Mykosen, 26: 145–151.
BennettS.C.J. (1931): Cryptococcus pneumonia in Equidae. - J. Comp. Pathol. Therap., 44: 85–105.
KapurJ.D. (1952): Epizootic lymphangitis. - Ind. Vet. J., 28: 342–343.
SinghT. (1965): Studies on epizootic lymphangitis. I. Mode of infection and transmission of equine histoplasmosis (epizootic lymphangitis). - Ind. J. Vet. Sci., 37: 47–57.
FaccincaniF. (1952): Multiple pulmonary nodules in epizootic lymphangitis. - Arch. Vet., Ital. 3: 27–32.
FawiM.T. (1971): Histoplasma farciminosum, the aetiological agent of equine cryptococcal pneumonia.- Sabouraudia, 9: 123–125.
AjelloL. (1968): Comparative morphology and immunology of members of the genus Histoplasma, Mykosen, 11: 507–514.
KhaterA.R., EskanderM. and MostafaA. (1968): A histopathological study of cutaneous lesions in equine histoplasmosis (epizootic lymphangitis). -J. Egypt. Vet. Med. Assoc., 28: 165–174.
RefaiM. and LootA. (1970): Incidence of epizootic lymphangitis in Egypt with reference to its geographical distribution. - Mykosen, 13: 247–252.
FouadK., SalehM.S., SokkerS. and ShoumanM.T. (1973): Studies on lachrymal histoplasmosis in donkeys in Egypt. - Zbl, Vet. Med., 20: 584–593.
PaneL. and DrouhetE. (1963): Sur l'obtention et la conservation de la. phase ievure d'Histoplasma capsulatum e du H. duboisii en milieu chimiquement defini. - Ann. Inst. Pasteur, 105: 798–804.
HuturaF., MarekJ. and ManningerR. (1946): Special Pathology and Therapeutics of the Diseases of Domestic Animals., pp. 779. Bailliere, Tindall and Cox, London.
BuxtonA. and FraserG. (1977): Animal Microbiology Vol. 1, Blackwell Scientific Publications, Oxford.
MarconeG. (1895): La saccaromicosi degli equini (farcino d'Africa, linfangite epizootica, farcino criptococchico ecc.). - Atti R. Ist. D'Incorrag. Napoli, 8: 1–19.
LamikovK.I. (1966): Concerning the cultivation of the causative agent of epizootic lymphangitis of horses (In Russian). - Veterinariya (Moskva), 23: 15–17.
BierbaumK. (1919): Uber die Zuchtung des Cryptococcus farciminosus (Rivolta). - Berliner Tierarztliche Wochenschrift, 26: 217–218.
EberbeckE. (1926): Aetiologisch-biologische und pathologisch-histologische Untersuchungen uber die Lymphangitis epizootica des Pferdes. - Arch. Wiss. Prakt. f. Tierbeilk., 54: 1–31.
PallinW.A. (1904): A Treatise on Epizootic Lymphangitis. 2nd Ed. Univ. Press of Liverpool, London.
HenningM.W. (1956): Animal Diseases in South Africa p. 196. Central News Agency, Ltd., South Africa.
Author information
Authors and Affiliations
Additional information
Corresponding author.
Rights and permissions
About this article
Cite this article
Selim, S.A., Soliman, R., Osman, K. et al. Studies on histoplasmosis farciminosi (epizootic lymphangitis) in Egypt. Eur J Epidemiol 1, 84–89 (1985). https://doi.org/10.1007/BF00141797
Issue Date:
DOI: https://doi.org/10.1007/BF00141797