Summary
An arylsulfotransferase from Eubacterium sp. was immobilized on agarose gels by multipoint covalent attachment. The yield of immobilization was 80% with an activity of 11 UA/ml of derivative. After 19 days of incubation, the loss of activity of the derivative was 36%. The immobilized preparation was used to transfer selectively a sulfate group from p-nitrophenolsulfate to selected tyrosine containing biologically active peptides in 92–99% of yield.
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Alvaro, G., Fernandez-Lafuente, R., Blanco, R.M., Guisán, J.M. (1990). Appl. Biochem. Biotechnol. 26, pp181–195.
Clapés, P., Torres, J.L., Adlercreutz, P. (1995) Bioorgan. Med. Chem. 3, 245–255.
East, I.J.; Dean, J. J. (1984). Cell Biol. 98, 795–800.
Hortin, G.L.; Farries, T.L.; Graham, J.P.; Atkinson, J.P.(1989). Proc. Nat. Acad. Sciences, U.S.A. 86, 1138–1142.
Guisán, J.M. (1988) Enzyme Microb. Technol. 10, 375–382.
Huttner, W.B. (1987) Trends Biochem. Sciences 12,361–363.
Kobashi, K., Kim, D.-H. (1986). Biochem. Biophys. Res. Commun. 140, 38–42.
Kobashi, K.; Kim, D.-H.; Fukaya, Y.; Akao, T.; Takebe, S. (1986). Arch. Biochem. Biophys. 246, 2, 537–539.
Kobashi, K. Kim, D.-H.; Morikawa, T. (1987). J. Prot. Chem. 6(3), 237–244.
Kim, D.-H., Konishi, L., Kobashi, K. (1986). Biochim. Biophys. Acta 872, 33–41.
Lin, C.H., Shen, G.J., García-Junceda, E., Wong, C.-H. (1995). J. Am Chem.Soc 117, 8031–8032.
Lin, W.-H.; Larsen, K.; Hortin, G.L.; Roth, J.A. (1992). J. Biol. Chem. 267, 2876–2879.
Mulder, G.J.; Jakoby, W.B. (1990). Sulfation. In: Conjugation Reactions in Drug Metabolism, G.J. Mulder ed., pp. 107–161 Taylor & Francis Ltd, United Kingdom.
Niehrs, L., Huttner, W.B., Carvallo, D., Degryse, I. (1990). J. Biol. Chem. 265, 9314–9318.
Penke, B.; Hajnal, F.; Lonovics, J.; Holzinger, G.; Kadar, T.; Telegdy, G.; Rivier, J. (1984). J. Med. Chem. 27, pp845–849
Tóth, G.K., Penke, B., Zarándi, M., Kovács, K. (1985) Int. J. Peptide Protein Res. 26, 630–638.
Yuen, C.-T., Bezouska, K., O'Brien, J., Stoll, M., Lemoine, R., Lubineau, A., Kiso, M., Hasegawa, A., Bochovich, J.N., Nicolaou, K.C., Feizi, T. (1994). J. Biol Chem. 269, 1595–1598.
Wieland, T., Penke, B. Birr, C. (1972). Liebigs Ann. Chem. 759, 71–75.
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Delhom, B., Alvaro, G., Caminal, G. et al. Synthesis of sulfated bioactive peptides using immobilized arylsulfotransferase from Eubacterium sp.. Biotechnol Lett 18, 609–614 (1996). https://doi.org/10.1007/BF00140212
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DOI: https://doi.org/10.1007/BF00140212