Skip to main content
Log in

Patterns of puffing activity and chromosomal polymorphism in Drosophila subobscura II. Puffing patterns at the prepupa stage

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

Puffing activity patterns of the five large polytene chromosomes of Drosophila subobscura were studied during the late third-larval instar and through the prepupal period. A total of 166 loci active in some of the eleven stages studied were described. The distribution of these active loci per chromosome is the following: 25 on chromosome A, 33 on chromosome J, 31 on chromosome U, 34 on chromosome E and 43 on chromosome O. Seven principal patterns of puffing activity were defined taking into account the different curves of the puffing histograms. Gene activities per chromosome as well as total were analysed. Three peaks of gene activity at the beginning, middle and ending of prepupation can be observed. U is the most active chromosome and A (the sex chromosome), and J the least active. Chromosomes E and O show a medium activity. A possible biological explanation for these results is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ashburner, M., 1967. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. I. Autosomal puffing patterns in a laboratory stock of Drosophila melanogaster. Chromosoma 21: 398–428.

    Google Scholar 

  • Ashburner, M., 1969a. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. II. X chromosome puffing patterns in Drosophila melanogaster and Drosophila simulans. Chromosoma 27: 47–63.

    Google Scholar 

  • Ashburner, M., 1969b. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. III. A comparison of the autosomal puffing patterns of the sibling species Drosophila melanogaster and Drosophila simulans. Chromosoma 27: 64–85.

    Google Scholar 

  • Ashburner, M., 1972. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. VI. Induction by ecdysone in salivary glands of Drosophila melanogaster cultured in vitro. Chromosoma 38: 255–281.

    Google Scholar 

  • Becker, H. J., 1959. Die Puffs der Speicheldrüsenchromosomen von Drosophila melanogaster. I. Beobachtungen zum Verhalten des Puffmusters im Normalstamm und bei zwei Mutanten giant und lethal-giant-larvae Chromosoma 10: 654–678.

    Google Scholar 

  • Becker, H. J., 1962. Die Puffs der Speicheldrüsenchromosomen von Drosophila melanogaster. II. Die Auslösung der Puffbildung, ihre Specifizität und ihre Beziehung zur Funktion der Ringdrüse. Chromosoma 13: 341–384.

    Google Scholar 

  • Beermann, W., 1982. Chromomerenkonstanz und spezifische Modifikationen der Chromosomenstruktur in der Entwicklung und Organdifferenzierung von Chironomus tentans. Chromosoma 5: 139–198.

    Google Scholar 

  • Berendes, H. D., 1965. Salivary gland function and chromosomal puffing patterns in Drosophila hydei. Chromosoma 17: 35–77.

    Google Scholar 

  • Berendes, H. D., 1967. The hormone ecdysone as effector of specific changes in the pattern of gene activities of Drosophila hydei. Chromosoma 22: 274–293.

    Google Scholar 

  • Breuer, M. E. & Pavan, C., 1955. Behaviour of polytene chromosomes of Rhynchosciara angelae at different stages of development. Chromosoma 7: 371–386.

    Google Scholar 

  • Clever, U., 1962. Genaktivitäten in den Riesenchromosomen von Chironomus tentans und ihre Beziehungen zur Entwicklung. II. Das Verhalten der Puffs während des letzten Larvenstadiums und der Puppenhäutung. Chromosoma 13: 385–436.

    Google Scholar 

  • DeFrutos, R. & Latorre, A., 1982a. Patterns of puffing activity in the polytene chromosomes of Drosophila subobscura. In: Lakkovaara, S. (ed.), Advances in genetics, development and evolution of Drosophila. Plenum Press, New York, pp. 33–46.

    Google Scholar 

  • DeFrutos, R. & Latorre, A., 1982b. Pattern of puffing activity and chromosomal polymorphism in Drosophila subobscura. I. J and U chromosomes. Genetica 58: 177–188.

    Google Scholar 

  • Kunze-Mühl, E. & Müller, E., 1958. Weitere Untersuchungen über die chromosomale Struktur und die natürlichen Strukturtypen von Drosophila subobscura Coll. Chromosoma 9: 559–570.

    Google Scholar 

  • Pascual, L., DeFrutos, R. & Latorre, A., 1983. Polytene chromosomes of Drosophila subobscura at the end of the prepupal stage. Drosoph. Inf. Serv. 59: 98–99.

    Google Scholar 

  • Silva, F. J., 1984, 3H-Uridine labelling patterns and chromosomal polymorphism in Drosophila subobscura: J and U chromosomes. Genetica (in press).

  • Stocker, A. J. & Kastritsis, C. D. 1972. Developmental studies in Drosophila. III. The puffing patterns of the salivary gland chromosomes of Drosophila pseudoobscura. Chromosoma 37: 139–176.

    Google Scholar 

  • Zhimulev, I. F. & Belyaeva, P. S., 1975. 3H-Uridine labelling patterns in the Drosophila melanogaster salivary gland chromosomes X, 2R and 3L. Chromosoma 49: 219–232.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Publication No. 107, Departamento de Genética, Universidad de Valencia (Spain).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pascual, L., De Frutos, R. & Latorre, A. Patterns of puffing activity and chromosomal polymorphism in Drosophila subobscura II. Puffing patterns at the prepupa stage. Genetica 66, 123–138 (1985). https://doi.org/10.1007/BF00139717

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00139717

Keywords

Navigation