Summary
The entrapment of Aspergillus ochraceus spores on to diatomaceous earth particles occurs rapidly, the number of spores entrapped at equilibrium being dependent upon the initial spore:particle ratio. The rate of agitation during spore uptake markedly affected entrapment. Immobilized spores carried out the 11α-hydroxylation of progesterone as effectively as free spores.
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Arcuri, E.J., Nichols, J.R., Brix, T.S., Santamarina, V.G., Buckland, B.C., and Drew, S.W. (1983). Biotechnol. Bioeng. 25, 2399–2411.
Gbewonyo, K., and Wang, D.I.C. (1983). Biotechnol. Bioeng. 25, 967–983.
Jaworski, A., Sedlaczek, L., Wilmanska, D., Sasiak, A., and Strycharska, A. (1982). Zeitschr.Allgem. Mikro. 22, 327–333.
Kim, M.N., Ergan, F., Dhulster, P., Atrat, P., Gellf, G., and Thomas, D. (1982). Biotechnol. Letts. 4, 233–238.
Maddox, I.S., Dunnill, P., and Lilly, M.D. (1981). Biotechnol. Bioeng. 23, 345–354.
Schleg, C., and Knight, S.G. (1962). Mycologia 54, 317–319.
Singh, K., Sehgal, S.N., and Vezina, C. (1968). Appl. Microbiol. 16, 393–400.
Sonomoto, K., Nomura, K., Tanaka, A., and Fukui, S. (1982). Eur. J. App. Microbiol. Biotechnol. 16, 57–62.
Vezina, C., Sehgal, S.N., and Singh, K. (1963). Appl. Microbiol. 11, 50–57.
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Broad, D.F., Foulkes, J. & Dunnill, P. The uptake of Aspergillus ochraceus spores on diatomaceous particles and their use in the 11α-hydroxylation of progesterone. Biotechnol Lett 6, 357–362 (1984). https://doi.org/10.1007/BF00138005
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DOI: https://doi.org/10.1007/BF00138005