Summary
Various microorganisms were screened for their ability to produce 2-ketobutyric acid (2-KBA) from 1,2-butanediol (1,2-BD). Among them, Rhodococcus equi IFO 3730 was selected as the best strain. The various culture and reaction conditions were optimized using this strain. Limitation of thiamine in the growing medium was found to be effective. The resting cells of the strain grown on 1,2-propanediol as the carbon source yielded 15.7 g/l of 2-KBA from 20 g/l of 1,2-BD after 32 h incubation at 30 °C in the reaction mixture under optimal conditions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
De Ley, J., and Kersters, K. (1964). Bacteriol. Rev., 28, 164–180.
Eggling, I., Cordes, C., Eggling, L., and Sahm, H. (1987). Appl. Microbiol. Biotechnol., 25, 346–351.
Harada, T., and Hirabayashi, T. (1968). Agric. Biol. Chem., 32, 1175–1180.
Kitahara, K., and Fukui, S. (1955). J. Gen. Appl. Microbiol., 1, 61–66.
Nakajima, T., Sawai, S., Sato, S., and Nakahara, T. (1994). Biosci. Biotech. Biochem., 58, in press.
Oogaki, M., Nakahara, T., Uchiyama, H., and Tahuchi, T. (1983). Agric. Biol. Chem., 47, 2619–2624.
Shigeno, T., Katayama A., and Nakahara T. (1992). Biosci. Biotech. Biochem., 56, 320–323.
Shigeno, T., and Nakahara T., (1991). Biotechnol. Lett., 13, 427–432.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nakahara, T., Nakajima-Kambe, T. & Sato, S. Production of 2-ketobutyric acid from 1,2-butanediol by resting cells of Rhodococcus equi IFO 3730. Biotechnol Lett 16, 263–268 (1994). https://doi.org/10.1007/BF00134623
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00134623