Abstract
In this work, γ-aminobutyric acid (GABA) was prepared by the decarboxylation of l-glutamate via l-glutamate decarboxylase in the resting cells of Lactobacillus brevis CGMCC No. 3414. The influence of cell concentration, cell age, buffer system, reaction time, and substrate concentration were investigated. The optimal composition of bioconversion system was composed of 50 g/L resting cells, cell age at 48 h fermentation, 0.2 M disodium hydrogen phosphate–citric acid buffer, and 25 mM monosodium glutamate. When the bioconversion system was performed at pH 4.6, 30°C, and 180 r/min shaking for 4 h, GABA production in biotransformation solution was 23.29 mM and the molar yield rate of bioconversion reached 93.15 %.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kinnersley AM, Turano FJ (2000) Gamma aminobutyric acid (GABA) and plant responses to stress. Crit Rev Plant Sci 19(6):479–509
Manyam BV, Katz L, Hare TA et al (1981) Isoniazid-induced elevation of CSF GABA levels and effects on chorea in Huntington’s disease. Ann Neurol 10(1):35–37
Yang S, Lu Z, Lu F et al (2005) Research progress on microbial glutamate decarboxylase. Food Sci 26(9):546–551
Okada T, Sugishita T, Murakami T et al (2000) Effect of the defatted rice germ enriched with GABA for sleeplessness, depression, autonomic disorder by oral administration. J Jpn Soc Food Sci 47(8):596–603
Omori M, Yano T, Okamoto J et al (1987) Effect of anaerobically treated tea (Gabaron tea) on blood pressure of spontaneously hypertensive rats. J Agr Chem Sci Jpn 61:1449–1451
Jones EA (2002) Ammonia, the GABA neurotransmitter system, and hepatic encephalopathy. Metab Brain Dis 17(4):275–281
Hagiwara H, Seki T, Ariga T (2004) The effect of pre-germinated brown rice intake on blood glucose and PAI-1 levels in streptozotocin-induced diabetic rats. Biosci Biotech Biochem (BBB) 68(2):444–447
DeFeudis FV (1983) γ-Aminobutyric acid and cardiovascular function. Experientia 39(8):845–849
Cavagnini F, Invitti C, Pinto M et al (1980) Effect of acute and repeated administration of gamma aminobutyric acid (GABA) on growth hormone and prolactin secretion in man. Endocrinol Acta 93(2):149–154
Zhao Y, Liang XL, Zhang H (2006) The Structure and function of the glutamate decarboxylase and its genes expression regulation in Escherichia coli. Food Ferment Ind 32(7):75–78
Peng C, Huang J, Hu S et al (2013) A two-stage pH and temperature control with substrate feeding strategy for production of gamma-aminobutyric acid by Lactobacillus brevis CGMCC 1306. Chinese J Chem Eng 21(10):1190–1194
Ueno H (2000) Enzymatic and structural aspects on glutamate decarboxylase. J Mol Catal B-Enzym 10(1):67–79
Battaglioli G, Liu H, Martin DL (2003) Kinetic differences between the isoforms of glutamate decarboxylase: implications for the regulation of GABA synthesis. J Neurochem 86(4):879–887
Gao Q, Duan Q, Wang D et al (2013) Separation and purification of γ-aminobutyric acid from fermentation broth by flocculation and chromatographic methodologies. J Agr Food Chem 61(8):1914–1919
Plokhov AY, Gusyatiner MM, Yampolskaya TA et al (2000) Preparation of γ-aminobutyric acid using E. coli cells with high activity of glutamate decarboxylase. Appl Biochem Biotech 88:257–265
Komatsuzaki N, Shima J, Kawamoto S et al (2005) Production of γ-aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods. Food Microbiol 22(6):497–504
Choi SI, Lee JW, Park SM et al (2006) Improvement of gamma-aminobutyric acid (GABA) production using cell entrapment of Lactobacillus brevis GABA 057. J Microbiol Biotech 16:562–568
Zhang Y, Song L, Gao Q et al (2012) The two-step biotransformation of monosodium glutamate to GABA by Lactobacillus brevis growing and resting cells. Appl Microbiol Biotech 94(6):1619–1627
Chen HM, Gao Q, Su Z et al (2012) Screening, identification and flask fermentation optimization of a high-yield γ-aminobutyric acid Enterococcus raffinosus strain. Microbiol China 39(11):1642–1652
Fu YX, Zhang T, Jiang B et al (2008) Enzymatic conversion for γ-aminobutyric acid by Lactococcus lactis. Sci Tech Food Ind 09:166–169
Satyanarayan V, Nair PM (1985) Purification and characterization of glutamate decarboxylase from Solanum tuberosum. Eur J Biochem 150(1):53–60
Nomura M, Nakajima I, Fujita Y (1999) Lactococcus lactis contains only one glutamate decarboxylase gene. Microbiology 145(6):1375–1380
Huang J, Mei LH (2007) Purification and characterization of glutamate decarboxylase of Lactobacillus brevis CGMCC 1306 isolated from fresh milk. Chinese J Chem Eng 15(2):157–161
Acknowledgments
This research was supported by the National 863 Program of China (2012AA021302), the National 973 Program of China (2014CB734004), and the National Natural Science Foundation of China (31370075 & 31471725).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Shi, Xf., Zheng, B., Chang, Cy., Cao, P., Yang, Hj., Gao, Q. (2015). Enzymatic Bioconversion for γ-Aminobutyric Acid by Lactobacillus brevis CGMCC No. 3414 Resting Cells. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_63
Download citation
DOI: https://doi.org/10.1007/978-3-662-46318-5_63
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-46317-8
Online ISBN: 978-3-662-46318-5
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)