Summary
β-Fructofuranosidase was immobilized by alginate gel at high efficiency (92 %). The extreme long-term continuous reaction (half-life, 275 days) was achieved by the immobilized enzyme using sucrose at high concentration (500 mg ml−1) to produce fructo-olicosaccharides, such as 1-kestose (Fruβ2→1Fruβ2→1aGlc) and nystose (Fruβ2→1Fruβ2→1Fruβ2→1aGlc).
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Hayashi, S., Tubouchi, M., Takasaki, Y. et al. Long-term continuous reaction of immobilized β-fructofuranosidase. Biotechnol Lett 16, 227–228 (1994). https://doi.org/10.1007/BF00134616
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DOI: https://doi.org/10.1007/BF00134616