Summary
An in vivo cloning system based on mini-Mu derivatives was used for cloning of E. coli penicillin G acylase gene (pac). We have constructed several recombinant clones producing penicillin G acylase and some of them exhibit approximately two times higher activity than original strains.
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Laczaová, A., Pechan, T., Stuchlík, S. et al. Cloning of E. coli penicillin G acylase gene with mini-Mu containing a plasmid replicon. Biotechnol Lett 17, 13–18 (1995). https://doi.org/10.1007/BF00134188
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DOI: https://doi.org/10.1007/BF00134188