Summary
The synthesis of fatty esters in aqueous medium by alcohoiysis catalysed by lipase from Candida parapsilosis (EC.3.1.1.3) is described. The transesterification of rapeseed oil with methanol leads to an equilibrium state after eight hours, with a yield (methyl esters formed/ total fatty acids initially present in the acylglycerols) of at least 53% (73, 45, 65% of the linolenic, linolek and oleic acids respectively). Yield was already about 42% after four hours of catalysis. This transesterification was permitted by methanol inhibition of hydrolysis of the esters.
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Briand, D., Dubreucq, E. & Galzy, P. Enzymatic fatty esters synthesis in aqueous medium with lipase from Candida parapsilosis (Ashford) Langeron and Talice. Biotechnol Lett 16, 813–818 (1994). https://doi.org/10.1007/BF00133959
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DOI: https://doi.org/10.1007/BF00133959