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Production of thermally induced recombinant proteins relative to cell biomass is influenced by cell density in Escherichia coli batch cultures

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Summary

Fed-batch cultures of recombinant Escherichia coli were studied regarding their ability to permit CI857ts-controlled gene expression at different growth phases. Cells, growing at 50% pO2 and 28°C in a bioreactor, were transferred to a shaker flask at 42°C and the product yield was analysed after a short incubation time. Within the batch period, the synthesis of recombinant β-galactosidase relative to the increase of biomass during the induction period was enhanced in an biomass-dependent fashion. Whereas in young cells biomass production dominates over recombinant protein production, the available energetic resources of aged cells seem to be employed more efficiently for CI857-controlled biosynthesis of recombinant proteins than of cell material. A model to describe this ‘altruistic’ behaviour of old cultures is here proposed.

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Authors and Affiliations

  1. Institut de Biologia Fonamental and Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain

    José Luis Corchero, Pau Vila & Antonio Villaverde

  2. Departament de Matemàtica Aplicada i Telemàtica, Universitat Politècnica de Catalunya, 08034, Barcelona, Spain

    Rafael Cubarsi

Authors
  1. José Luis Corchero
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  2. Pau Vila
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  3. Rafael Cubarsi
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  4. Antonio Villaverde
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Corchero, J.L., Vila, P., Cubarsi, R. et al. Production of thermally induced recombinant proteins relative to cell biomass is influenced by cell density in Escherichia coli batch cultures. Biotechnol Lett 16, 777–782 (1994). https://doi.org/10.1007/BF00133953

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  • DOI: https://doi.org/10.1007/BF00133953

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