Skip to main content
SpringerLink
Log in

The influence of heat shock on the puffing pattern of Drosophila auraria polytene chromosomes

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

The influence of heat shock on the puffing pattern of salivary gland polytene chromosomes of D. auraria from five developmental stages was investigated. Under heat shock conditions, seven puffs appear to be transcriptionally active, five of which are active during normal development as well. Two sublines of D. auraria, differing in the activity of one of the Balbiani rings known to exist in this species, also differ clearly in the size and the transcriptional activity of a puff (64B) which is induced by heat shock in one of the sublines, but not in the other; the polytene chromosomes of the hybrids between the sublines do not exhibit this puff under heat shock conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alahiotis, S. N., 1983. Heat shoek proteins. A new view on the temperature compensation. Comp. Biochem. Physiol. 75B: 379–387.

    Google Scholar 

  • Ashburner, M., 1967. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. I. Autosomal puffing patterns in a laboratory stock of Drosophila melanogaster. Chromosoma 21: 398–428.

    Google Scholar 

  • Ashburner, M., 1970. Patterns of Puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments. Chromosoma 31: 356–376.

    Google Scholar 

  • Ashburner, M. & Bonner, J. J., 1979. The induction of gene activity in Drosophila by heat shock. Cell 17: 241–254.

    Google Scholar 

  • Becker, H.-J., 1959. Die Puffs der Speicheldrüsenchromosomen von Drosophila melanogaster. I. Beobachtungen zum Verhalten des Puffmusters im Normalstamm und bei zwei Mutanten, giant und lethal-giant larvae. Chromosoma 10: 654–678.

    Google Scholar 

  • Berendes, H. D., 1968. Factors involved in the expression of gene activity in polytene chromosomes. Chromosoma 24: 418–437.

    Google Scholar 

  • Berendes, H. D., Van Breugel, F. M. A. & Holt, Th. K. H., 1965. Experimental puffs in salivary gland chromosomes of Drosophila hydei. Chromosoma 16: 35–46.

    Google Scholar 

  • Bonner, J. J. & Kerby, R. L., 1982. RNA polymerase II transcribes all of the heat shock induced genes of Drosophila melanogaster. Chromosoma 85: 93–108.

    Google Scholar 

  • Gausz, J., Bencze, G., Gyurkovics, H., Ashburner, M., Ish-Horowicz, D. & Holden, J. J., 1979. Genetic characterization of the 87C region of the third chromosome of Drosophila melanogaster. Genetics 93: 917–934.

    Google Scholar 

  • Gausz, J., Gyurkovies, H., Benze, G., Rwad, A., Holden, J. & Ish-Horowicz, D., 1981. Genetic characterization of the region between 86F1,2 and 87B15 on chromosome 3 of Drosophila melanogaster. Genetics 98: 775–789.

    Google Scholar 

  • Greenleaf, A. L., Plagens, U., Jamrich, M. & Bautz, E. K. F., 1978. RNA polymerase B (or II) in the heat induced puffs of Drosophila polytene chromosomes. Chromosoma 65: 127–136.

    Google Scholar 

  • Holmgren, R., Livak, L., Morimoto, R., Freund, R. & Meselson, M., 1979. Studies of cloned sequences from four Drosophila heat shock loci. Cell 18: 1359–1370.

    Google Scholar 

  • Ish-Horowicz, D. & Pinchin, S. M., 1980. Genomic organization of the 87A7 and 87C1 heat induced loci of Drosophila melanogaster. J. mol. Biol. 142: 231–245.

    Google Scholar 

  • Jamrich, M., Greenleaf, A. L. & Bautz, E. K. F., 1977. Localization of RNA polymerase in polytene chromosomes of Drosophila melanogaster. Proc. natn. Acad. Sci. U.S.A. 74: 2079–2083.

    Google Scholar 

  • Kastritsis, C. D. & Grossfield, J., 1971. Balbiani rings in D. auraria. Dros. Inf. Serv. 47: 123.

    Google Scholar 

  • Lewis, M., Helmsing, P. J. & Ashburner, M., 1975. Parallel changes in puffing activity and protein synthesis in salivary glands of Drosophila. Proc. natn. Acad. Sci. U.S.A. 72: 3604–3608.

    Google Scholar 

  • Mohler, J. & Pardue, M. L., 1982. Deletion mapping of the 93D heat shock locus of Drosophila melanogaster. Chromosoma 86: 457–467.

    Google Scholar 

  • Mohler, J. & Pardue, M. L., 1983. Mutational analysis of the region surrounding the 93D heat shock locus of Drosophila melanogaster. Genetics 106: 249–265.

    Google Scholar 

  • Pardue, M. L. & Gall, J. G., 1975. Nucleic acid hybridization to the DNA of cytological preparations. In: Methods in cell biology (D. M. Prescott, ed.) vol. X, pp. 1–16, Academic Press, New York.

    Google Scholar 

  • Ritossa, F. M., 1962. A new puffing pattern induced by temperature shock and DNP in Drosophila. Experientia 18: 571–573.

    Google Scholar 

  • Ritossa, F. M., 1963. New puffs induced by temperature shock, DNP and salicilate in salivary gland ehromosomes of D. melanogaster. Dros. Inf. Serv. 37: 122–123.

    Google Scholar 

  • Ritossa, F. M., 1964. Experimental activation of specific loci in polytene chromosomes of Drosophila. Expl. Cell Res. 35: 601–607.

    Google Scholar 

  • Scouras, Z. G., 1984. Study of the salivary gland polytene chromosomes of Drosophila auraria Doct. Dissertation. Scient. Ann. School Sci., Univ. of Thessaloniki, pp. 1–159.

  • Scouras, Z. G. & Kastritsis, C. D., 1984. Balbiani rings and puffs of the polytene chromosomes of Drosophila auraria. Chromosoma 89: 96–106.

    Google Scholar 

  • Selleck, S. B., Elgin, S. S. R. & Cartwright, I. L., 1984. Supercoll-dependent features of DNA structure at Drosophila locus 67B1. J. Mol. Biol. 178: 17–33.

    Google Scholar 

  • Semeshin, V. G., Baricheva, E. M., Belyaeva, E. S. & Zhimulev, I. F., 1982. Electron microscopical analysis of Drosophila polytene chromosomes. I. Mapping of the 87A and 87C heat shock puffs in development. Chromosoma 87: 229–237.

    Google Scholar 

  • Southgate, R., Ayme, A. & Voelimy, R., 1983. Nucleotide sequence analysis of the Drosophila small heat shock gene eluster at locus 67B. J. mol. Biol. 165: 35–57.

    Google Scholar 

  • Tanguay, R. M., 1983. Genetic regulation during heat shock and function of heat shock proteins: a review. Can. J. Biochem. Cell Biol. 61: 387–394.

    Google Scholar 

  • Tissieres, A., Mitchell, H. K. & Tracy, U. M., 1974. Protein synthesis in salivary glands of D. melanogaster, Relation to chromosome puffs. J. mol. Biol. 84: 389–398.

    Google Scholar 

  • Velasquez, J. M., DiDomenico, B. J. & Lindquist, S., 1980. Intracellular localization of heat shock proteins in Drosophila. Cell 20: 679–689.

    Google Scholar 

  • Voelimy, R., Goldsehmidt-Clermont, M., Southgate, R., Tissieres, A., Levis, R. & Gehring, W., 1981. A DNA segment isolated from chromosomal site 67B in D. melanogaster contains four closely linked heat shock genes. Cell 23: 261–270.

    Google Scholar 

  • Zhimulev, I. F., Belyaeva, E. S. & Semeshin, V. F., 1981. Informational content of polytene chromosome bands and puffs. CRC Crit. Rev. Biochem. 11: 303–340.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, School of Sciences, Aristolelian University of Thessaloniki, Grece

    Z. G. Scouras, G. A. Karamaplidou & C. D. Kastritsis

Authors
  1. Z. G. Scouras
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. A. Karamaplidou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. D. Kastritsis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scouras, Z.G., Karamaplidou, G.A. & Kastritsis, C.D. The influence of heat shock on the puffing pattern of Drosophila auraria polytene chromosomes. Genetica 69, 213–218 (1986). https://doi.org/10.1007/BF00133524

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00133524

Keywords

Search

Navigation