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High level expression of a thermostable Bacillus xylose (glucose) isomerase in Escherichia coli

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Summary

A thermophilic Bacillus sp. producing xylose (glucose) isomerase has been isolated. Its xy/A gene when cloned in Escherichia coli and expressed gave 37.5 and 12.8 units/ mg protein respectively for xylose and glucose isomerase activities at 85°C. A single heat treatment of the crude extract purified the enzyme further yielding the highest ever recorded activities of 150 and 49.02 units /mg protein.

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References

  • Anagnostopoulos, C. and Spizizen, J. (1961) J. Bacteriol. 81, 741–746.

    Google Scholar 

  • Chen, W.P. (1980) Process Biochem. 15, 30–41.

    Google Scholar 

  • Dengen, L., Oddo, N. and Olivieri, R. (1974) German Patent 2,413,939.

  • Dekker, K., Yamagata, H. Sakaguchi, K. and Udaka, S. (1991) J. Bacteriol. 173, 3078–3083.

    Google Scholar 

  • Diers, I.V. and Vaerloese, L. (1977) U.S. Patent 4,042,461.

  • Dische, Z. and Borenfreund, E (1951) J. Biol. Chem. 191, 583–587.

    Google Scholar 

  • Feldmann,S.D., Sahm,H. and Sprenger,G.A. (1992) Mol. Gen. Genet. 234, 201–210.

    Google Scholar 

  • Horecker, B.L. (1974) Methods of Enzymatic Analysis. Bergmeyer H.U., ed. pp. 1371–1376. New York: Academic Press.

    Google Scholar 

  • Jeyaseelan, K., Singh, P., Thirumaran, T. and Wong, H.A. (1991) Microbiol utilization of renewable resources. Kinoshita S. and Bhumiratna A., eds. 7, 200–208. Japan: Osaka University Press.

    Google Scholar 

  • Karbassi, A. and Vaughn, R.H. (1980) Can. J. Microbiol. 26, 377–384.

    Google Scholar 

  • Lee, C., Bhatnagar, L., Saha, B.C., Lee, Y., Takagi, M., Imanaka, T., Bagdasarian, M. and Zeikus, J.G. (1990) Appl. Environ. Microbiol. 56, 2638–2643.

    Google Scholar 

  • Outtrup, H. Vaerloese (1976) (1976) U.S. Patent 3,979,261.

    Google Scholar 

  • Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989): Molecular Cloning: A laboratory manual, vol 1–3, New York: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  • Suekane, M., Kanno, S. and M. and Hasegawa, S. (1974) U.S. Patent 3,826,714.

  • Wilhelm, M. and Hollenberg, C.P. (1985) Nucleic Acids Res. 13, 5717–5722.

    Google Scholar 

  • Wong, H.C., Ting, Y., Lin, H.C., Reichert, F., Myambo, K., Watt, K.W.K., Toy, P.L. and Drummond, R.J. (1991) J. Bacteriol. 173, 6849–6858.

    Google Scholar 

  • Yamanaka, K. (1968) Biochem. Biophys. Acta 151, 670–680.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 0511, Singapore, Singapore

    Liao Wuxiang & Kandiah Jeyaseelan

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  1. Liao Wuxiang
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  2. Kandiah Jeyaseelan
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Wuxiang, L., Jeyaseelan, K. High level expression of a thermostable Bacillus xylose (glucose) isomerase in Escherichia coli . Biotechnol Lett 15, 1101–1106 (1993). https://doi.org/10.1007/BF00131197

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  • DOI: https://doi.org/10.1007/BF00131197

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