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Growth temperature is involved in the regulation of extracellular lipase at two different levels in Pseudomonas fluorescens strain MF0

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Summary

At chill temperatures, Pseudomonas fluorescens produces inducible extracellular protease and lipase. Their regulation by growth temperature and conditions was studied in chemostat experiments and by the use of a transcriptional fusion in the structural lipase gene. The findings suggest two levels of temperature regulation of lipase production, resulting in maximal activity at about 17°C, well below the optimum for growth.

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References

  • Andersson, R.E. (1980). Appl. Env. Microbiol. 39, 36–40.

    Google Scholar 

  • Andrews, B.A. and Asenjo, J.A. (1987). Biotechnol. Bioeng. 30, 628–637.

    Google Scholar 

  • Burini, J.F., Gügi, B., Meneau, A. and Guespin-Michel, J.F. (1994). FEMS Microbiol. Lett. 122, 13–18.

    Google Scholar 

  • Duong, F. Soscia, C. Ladzunshi, A. and Murgier, M. (1994). Mol. Microbiol. 11, 1117–1126.

    Google Scholar 

  • Fairbairn, D.J. and Law, B.A. (1987). J. Appl. Bacteriol. 62, 105–113.

    Google Scholar 

  • Greenman, J. and Holland, T. (1985). J. Gen. Microbiol. 131, 1619–1624.

    Google Scholar 

  • Gügi, B., Orange, N., Hellio, F., Burini, J.F., Guillou, C., Leriche, F. and Guespin-Michel, J.F. (1991). J. Bacteriol. 173, 3814–3820.

    Google Scholar 

  • Hellio, F., Orange, N. and Guespin-Michel, J.F. (1993). Res. Microbiol. 144, 617–625.

    Google Scholar 

  • Jacques, N.A., Jacques, V.L., Wolf, A.C. and Wittenberg,C.L. (1985). J. Gen. Microbiol. 131, 67–72.

    Google Scholar 

  • Merieau, A., Gügi, B., Guespin-Michel, J.F. and Orange, N. (1993). Appl. Microbiol. Biotechnol. 39, 104–109.

    Google Scholar 

  • Miller, J.H. (1972). In: Experiments in Molecular Genetics. Cold Spring Harbor, eds. pp 352–355. New York; Laboratory Press.

    Google Scholar 

  • Rouwenhorst, R.J., Visser, L.E., Van Der Baan, A.A., Scheffers, W.A. and Van Dijken, J.P. (1988). Appl. Environ. Microbiol. 54, 1131–1137.

    Google Scholar 

  • Smeltzer, M.S., Hart, M.E. and Iandolo, J.J. (1992). Appl. Environ. Microbiol. 58, 2815–2819.

    Google Scholar 

  • Stepaniak, L. and Fox, P. (1985). J. Dairy Res. 52, 77–89.

    Google Scholar 

  • Tan, K.H. and Gill, C.O. (1987). Appl. Microbiol. Biotechnol. 26, 443–446

    Google Scholar 

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Guillou, C., Merieau, A., Trebert, B. et al. Growth temperature is involved in the regulation of extracellular lipase at two different levels in Pseudomonas fluorescens strain MF0. Biotechnol Lett 17, 377–382 (1995). https://doi.org/10.1007/BF00130793

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  • DOI: https://doi.org/10.1007/BF00130793

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