Skip to main content
SpringerLink
Log in

Synthesis of L-tyrosine glyceryl ester catalyzed by α-chymotrypsin in water-miscible organic solvents: A possible sun-tan accelerator product

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

The synthesis of L-tyrosine glyceryl ester, from glycerol and L-tyrosine methyl ester, was carried out by a transesterification reaction catalyzed by α-chymotrypsin. Values of 60 % (v/v) for glycerol and 200 mM for L-tyrosine methyl ester were optimal for the transesterification reaction. Additionally to glycerol, several other water miscible cosolvents (acetonitrile, N,N'-dimetyl formamide and tetrahydrofurane) were tested in the reaction media, but their presence did not give an enhancement on the transesterification activity with respect to the glycerol/water medium. However, increasing the hydrophobicity of the cosolvent resulted in a reduction of the enzyme activity, the water:glycerol mixture being the best reaction media.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Cardillo-Theobaldo, F., Liru, E., Chang, E., Irokawa, A. and Tominaga, M. (1991). Biotechnol. Tech., 5, 73–76.

    Google Scholar 

  • Combes, D. and Lozano, P. (1992). Ann. N.Y. Acad. Sci., 672, 409#x2013;414.

    Google Scholar 

  • Ingalls, R.G., Squires, R.G. and Butler, L.G. (1975). Biotechnol. Bioeng., 17, 1627–1637.

    Google Scholar 

  • Kise, H. and Hayakawa, A. (1991). Enzyme Microb. Technol., 13, 584–588.

    Google Scholar 

  • Kise, H., Hayakawa, A. and Noritomi H. (1990). J. Biotechnol., 14, 239–254.

    Google Scholar 

  • Lozano, P. and Combes, D. (1992). Appl. Biochem. Biotechnol., 33, 51–65.

    Google Scholar 

  • Lozano, P. Iborra, J.L., Manjón, A. and Combes, D. (1992). Biotechnol. Lett., 14, 933–936.

    Google Scholar 

  • Monsan, P. and Paul, F. (1990). New development in the application of enzyme catalysis to synthesis reactions. In: Proceedings of the 5th European Congress on Biotechnology. C. Christiansen, L. Munk and J. Villadsen, eds. vol. 1, pp. 233–238. Copenhagen: Munksgaard.

    Google Scholar 

  • Mori, T., Nilsson, K., Larsson, P.O. and Mosbach, K. (1987). Biotechnol. Lett., 9, 455–460.

    Google Scholar 

  • Phillips, R.S., Mattews, M.S., Olson, E. and von Tersch, R.L. (1990). Enzyme Microb. Technol., 12, 731–735.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Bioquímica y Biología Molecular B e Inmunología. Facultad de Química, Universidad de Murcia, Ap. 4021, E-30001, Murcia, España

    Pedro Lozano, Didier Combes, José L. Iborra & Arturo Manjón

  2. Département de Génie Biochimique et Alimentaire, INSA. UA-CNRS 544. Complexe Scientifique de Rangueil, F-31077, Toulouse, France

    Didier Combes

Authors
  1. Pedro Lozano
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Didier Combes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. José L. Iborra
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Arturo Manjón
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lozano, P., Combes, D., Iborra, J.L. et al. Synthesis of L-tyrosine glyceryl ester catalyzed by α-chymotrypsin in water-miscible organic solvents: A possible sun-tan accelerator product. Biotechnol Lett 15, 1223–1228 (1993). https://doi.org/10.1007/BF00130301

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00130301

Keywords

Search

Navigation