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Glycerolysis of tallow with immobilised lipase

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Summary

Lipozyme (immobilised Mucor meihei lipase) was used to catalyse glycerolysis of melted tallow to synthesise monoglycerides. At a reaction temperature of 50 °C, a maximum 35% yield of monoglyceride was obtained. Cooling the reaction below 42°C resulted in monoglyceride crystallisation which improved the yield to up to 50% but further yield increases were prevented by solidification of the reaction mixture. Enzyme could be easily recovered by filtration after melting the reaction mixture. The reaction could also be carried out by circulating the reaction mixture through a packed column of lipozyme.

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Authors and Affiliations

  1. Industrial Research Ltd, PO Box 31-310, Lower Hutt, New Zealand

    David E Stevenson, Roger A Stanley & Richard H Fumeaux

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  1. David E Stevenson
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  2. Roger A Stanley
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  3. Richard H Fumeaux
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Stevenson, D.E., Stanley, R.A. & Fumeaux, R.H. Glycerolysis of tallow with immobilised lipase. Biotechnol Lett 15, 1043–1048 (1993). https://doi.org/10.1007/BF00129935

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