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Effect of cell-cycle inhibition on synthesis of steroidal alkaloids by Solanum aviculare plant cells

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Summary

Addition of 40 mg l-1 hydroxyurea to Solanium aviculare cultures resulted in complete inhibition of growth and an increase in the levels of steroidal alkaloids in the biomass. These results demonstrate that solasodine synthesis and growth can be uncoupled, and suggest that steroidal-alkaloid production can be significantly enhanced by inhibiting growth at high cell density. Treatment with 1 mg l-1 abscisic acid had a negligible effect on growth and product synthesis.

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References

  • Chandler, S. and Dodds, J. (1983). Plant Cell Rep. 2, 69–72.

    Google Scholar 

  • Eriksson, T. (1966). Physiol. Plant. 19, 900–910.

    Google Scholar 

  • Khanna, P., Uddin, A., Sharma, G.L., Manot, S.K. and Rathore, A.K. (1976). Ind. J. Exp. Biol. 14, 694–696.

    Google Scholar 

  • Komamine, A., Sakuta, M., Hirose, M., Hirano, H., Takagi, T., Kakegawa, K. and Ozeki, Y. (1989). Regulation of secondary metabolism in relation to growth and differentiation. In: Primary and Secondary Metabolism of Plant Cell Cultures II, W.G.W. Kurz, ed, pp. 49–52, Berlin: Springer.

    Google Scholar 

  • Macek, T.E. (1989). Solanum aviculare Forst., Solanum laciniatum Ait (poroporo): in vitro culture and the production of solasodine. In: Biotechnology in Agriculture and Forestry, Y.P.S. Bajaj, ed, vol 7, pp. 443–467, Berlin: Springer.

    Google Scholar 

  • Nigra, H.M., Caso, O.H. and Giulietti, A.M. (1987). Plant Cell Rep. 6, 135–137.

    Google Scholar 

  • Rosenkranz, H.S., Garro, A.J., Levy, J.A. and Carr, H.S. (1966). Biochim. Biophys. Acta 114, 501–515.

    Google Scholar 

  • Tsoulpha, P. and Doran, P.M. (1991). J. Biotechnol. 19, 99–110.

    Google Scholar 

  • Yeoman, M.M., Miedzybrodzka, M.B., Lindsey, K. and McLauchlan, W.R. (1980). The synthetic potential of cultured plant cells. In: Developments in Plant Biology, F. Sala, B. Parisi, R. Cella and O. Ciferri, eds, vol 5, pp. 327–343, Amsterdam: Elsevier.

    Google Scholar 

  • Yeoman, M.M., Lindsey, K., Miedzybrodzka, M.B. and McLauchlan, W.R. (1982). Accumulation of secondary products as a facet of differentiation in plant cell and tissue cultures. In: Differentiation In Vitro, M.M. Yeoman and D.E.S. Truman, eds, pp. 65–82, Cambridge: Cambridge University Press.

    Google Scholar 

  • Young, C.W., Schochetman, G. and Karnofsky, D.A. (1967). Cancer Res. 27, 526–534.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, University of New South Wales, PO Box 1, 2033, Kensington, NSW, Australia

    Yunxian Mak & Pauline M. Doran

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  1. Yunxian Mak
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  2. Pauline M. Doran
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Mak, Y., Doran, P.M. Effect of cell-cycle inhibition on synthesis of steroidal alkaloids by Solanum aviculare plant cells. Biotechnol Lett 15, 1031–1034 (1993). https://doi.org/10.1007/BF00129932

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