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Activation of mushroom polyphenol oxidase in organic medium by the detergent SDS

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Summary

Polyphenol oxidase in chloroform was shown to have increased catalytic efficiency, and to transform sterically more demanding substrates, in the presence of SDS, compared with its absence. The limited protein flexibility due to chloroform were apparently altered by the SDS, with little loss of activity.

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Rhodes University, 6140, Grahamstown, South Africa

    Stephanie G. Burton & John R. Duncan

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  1. Stephanie G. Burton
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  2. John R. Duncan
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Burton, S.G., Duncan, J.R. Activation of mushroom polyphenol oxidase in organic medium by the detergent SDS. Biotechnol Lett 17, 627–630 (1995). https://doi.org/10.1007/BF00129390

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  • DOI: https://doi.org/10.1007/BF00129390

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