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Metabolic enhancement due to plasmid maintenance

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Summary

Plasmid maintenance allows the strain JM109 of Escherichia coli to grow in a minimal defined medium (M9). JM109 carrying no plasmid can hardly grow in M9 whereas JM109 carrying one, two and three plasmids have a clear metabolic advantage over the untransformed strain. In a complex medium like LB (Luria-Bertani Broth) all strains grow well and despite the number of plasmids carried by the host maximum specific growth rates are not severely affected. Our results suggest that the glucose metabolism is an essential factor contributing to this behavior.

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Authors and Affiliations

  1. Institut für Technische Chemie, Universität Hannover, Callinstr. 3, D-30167, Hannover, Germany

    Jong Il Rhee, Juan C. Diaz Ricci, Jens Bode & Karl Schügerl

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  1. Jong Il Rhee
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  2. Juan C. Diaz Ricci
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  3. Jens Bode
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  4. Karl Schügerl
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Il Rhee, J., Diaz Ricci, J.C., Bode, J. et al. Metabolic enhancement due to plasmid maintenance. Biotechnol Lett 16, 881–884 (1994). https://doi.org/10.1007/BF00128618

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  • DOI: https://doi.org/10.1007/BF00128618

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