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CMCase production by Spicellum roseum in liquid and solid culture

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Summary

CMCase was produced by 7 strains of Spicellum roseum in both liquid and wheat bran solid substrate cultures. No growth occurred above 35°C. Maximum enzyme production occurred at 30°C, whereas best enzyme activity occurred at pH 5.0 and 50°C. In liquid cultures of S. roseum, NRRL strains 13103, 13104, and 13106 produced activities of ca. 1.1, 1.5, and 1.5 mg glucose per hr/ml culture supernate at 1 week and 2.9, 1.5, and 2.1, respectively at 3 weeks compared to Trichoderma reesei NRRL 11236 (MCG77), which produced activities of 2.8 and 1.3 at 1 and 3 weeks.

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Authors and Affiliations

  1. U.S. Department of Agriculture, Northern Regional Research Center, Agricultural Research Service, 61604, Peoria, Illinois, USA

    Robert W. Silman, John E. McGhee & Rodney J. Bothast

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  1. Robert W. Silman
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  2. John E. McGhee
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  3. Rodney J. Bothast
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The mention of firm names or trade products does not imply that they are endorsed or recommended by the U.S. Department of Agriculture over other firms or similar products not mentioned.

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Silman, R.W., McGhee, J.E. & Bothast, R.J. CMCase production by Spicellum roseum in liquid and solid culture. Biotechnol Lett 6, 115–118 (1984). https://doi.org/10.1007/BF00127300

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  • DOI: https://doi.org/10.1007/BF00127300

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