Skip to main content
SpringerLink
Log in

The metaphase banding pattern produced in the chromosomes of Culiseta longiareolata (Diptera: Culicidae) by AluI restriction endonuclease or actinomycin-D treatment followed by acridine-orange staining

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

Cytological preparations of Culiseta longiareolata were treated with restriction endonuclease AluI or actinomycin-D and then stained with acridine orange. The longitudinal differentiation obtained was related to previous cytological data found in this species and interpreted as a possible consequence of the presence of satellite DNA in specific chromosome areas.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arrighi, F. E. & Hsu, T. C., 1971. Localization of heterochromatin.

  • Brown, S. W., 1966. Heterochromatin. Science 151: 417–425.

    Google Scholar 

  • Comings, D. E., 1978. Mechanisms of chromosome banding and implications for chromosome structure. A. Rev. Genet. 12: 25–46.

    Google Scholar 

  • Heitz, E., 1928. Das Heterochromatin der Moose. Jb. wiss. Bot. 69: 762–818.

    Google Scholar 

  • Jones, K. W. 1970. Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature 225: 912–915.

    Google Scholar 

  • Kuo, M. T., 1979. Studies in heterochromatin DNA: accessibility of late replicating heterochromatin DNA in chromatin to micrococcal nuclease digestion. Chromosoma 70: 183–194.

    Google Scholar 

  • Latt, S. A. & Wohlleb, J. C., 1975. Optical studies of the interactions of 33258 Hoechst with DNA, chromatin and metaphase chromosomes. Chromosoma 52: 297–316.

    Google Scholar 

  • Lin, C. C., Jorgenson, K. F. & Van de Sande, J. H., 1980. Specific fluorescent bands on chromosomes produced by Acridine-Orange after prestaining with base specific non-fluorescent ligands. Chromosoma 79: 271–286.

    Google Scholar 

  • Mazrimas, J. A., Balhorn, R. & Hatch, F. T., 1979. Separation of satellite DNA chromatin and main band DNA chromatin from mouse brain. Nucleic Acids Res. 7: 935–946.

    Google Scholar 

  • Mezzanotte, R., Ferrucci, L. & Contini, C., 1979a. Identification of sex chromosomes and characterization of the heterochromatin in Culiseta longiareolata (Macquart, 1838). Genetica 50: 135–139.

    Google Scholar 

  • Mezzanotte, R., Marchi, A. & Ferrucci, L., 1979b. The differential banding pattern produced by Hoechat 33258 and quinaerine dihydrochloride in metaphase chromosomes of Cullseta longiareolata (Dintera: Culieidae). Caryologia 32: 373–377.

    Google Scholar 

  • Mezzanotte, R., Ferrucci, L. & Marchi, A., 1979c. Light-induced banding (I.IB) in metaphase chromosomes of Culiseta longiareolata (Diptera: Culieidae). Genetiea 51: 140–152.

    Google Scholar 

  • Mezzanotte, R. & Ferrucci, L., 1981. The differential banding pattern produced by Actinomycin-D/Acridine-Orange counterstaining in metaphase chromosomes of Drosophila melanogaster. Experientia 37: 822–823.

    Google Scholar 

  • Mezzanotte, R., Ferrucci, L., Marchi, A. & Bianchi, U., 1982a. The longitudinal differentiation produced by photo-oxidation and acridine orange staining in sukaryote chromosomes: role and involvement of DNA base composition. Cytogenet. Cell Genet. 33: 277–284.

    Google Scholar 

  • Mezzanotte, R., Ferrucci, L., Vanni, R. & Bianchi, U., 1982b. The selective digestion of human metaphase chromosomes by AluI restriction endonucleasc. J. Histochem. Cytochem., in press.

  • Pardue, M. L. & Gall, J. G., 1970. Chromosomal localization of mouse satellite DNA. Science 168: 1356–1358.

    Google Scholar 

  • Pimpinelli, S., Santini, G. & Gatti, M., 1976. Characterization of Drosophila heterochromatin. II. C- and N-banding. Chromosoma 57: 377–386.

    Google Scholar 

  • Sahar, E. & Latt, S. A., 1980. Energy transfer and binding competition between dyes used to enhance staining differentiation in metaphase chromosomes. Chromosoma 79: 1–28.

    Google Scholar 

  • Sumner, A. T., 1982. The nature and mechanisms of chromosome banding. Cancer Genet. Cytogenet. 6: 59–87.

    Google Scholar 

  • Waring, M. J., 1968. Drugs which affect the structure and function of DNA. Nature 219: 1320–1325.

    Google Scholar 

  • Weisblum, B., 1973. Fluorescent probes of chromosomal DNA structure: three classes of acridines. Cold Spring Harb. Symp. quant. Biol. 38: 441–449.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Biologia Generale, Facoltà di Medicina e Chirurgia, Università, Via Ospedale 121, 09100, Cagliari, Italy

    R. Mezzanotte & L. Ferrucci

Authors
  1. R. Mezzanotte
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Ferrucci
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mezzanotte, R., Ferrucci, L. The metaphase banding pattern produced in the chromosomes of Culiseta longiareolata (Diptera: Culicidae) by AluI restriction endonuclease or actinomycin-D treatment followed by acridine-orange staining. Genetica 62, 47–50 (1983). https://doi.org/10.1007/BF00123309

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00123309

Keywords

Search

Navigation